LOCUS       sequence-91818-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKOet-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             128..778
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca agggcgagga
      121 gaataacatg gccgtgatta aaccagagat gaagatgagg tactacatgg acggctccgt
      181 caatgggcat gagttcacaa ttgaaggtga aggcacaggc agaccttacg agggacatca
      241 agagatgaca ctacgcgtca caatggccaa gggcgggcca atgcctttcg cgtttgactt
      301 agtgtcacac gtgttctgtt acggccacag accttttact aaatatccag aagagatacc
      361 agactatttc aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt tggagttcga
      421 agatggtggg tccgcttcag tcagtgcgca tataagcctt agaggaaaca ccttctacca
      481 caaatccaaa tttactgggg ttaactttcc tgccgatggt cctatcatgc aaaaccaaag
      541 tgttgattgg gagccatcaa ccgagaaaat tactgccagc gacggagttc tgaagggtga
      601 tgttacgatg tacctaaaac ttgaaggagg cggcaatcac aaatgccaat tcaagactac
      661 ttacaaggcg gcaaaaaaga ttcttaaaat gccaggaagc cattacatca gccatcgcct
      721 cgtcaggaaa accgaaggca acattactga gctggtagaa gatgcagtag ctcattccgg
      781 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
      841 catttgtaga ggtt
//
