LOCUS       sequence-91821-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-C1
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..746
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    747..812
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggaga
       61 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
      121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggctttcaga
      181 ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      241 ctcatttcac ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      301 tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaactac gaggacggcg
      361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      421 agctgcgcgg caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct
      481 gggaggcctc ctccgagcgg atgtaccccg aggacggtgc cctgaagggc aagatcaaga
      541 tgaggctgaa gctgaaggac ggcggccact acacctccga ggtcaagacc acctacaagg
      601 ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcacct
      661 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      721 ccggcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
      781 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      841 gccataccac atttgtagag gttttacttg ctttaa
//
