LOCUS       sequence-91822-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 548, Emission: 562.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mOrange-C1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..746
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     452..471
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    747..812
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggaga
       61 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
      121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggctttcaga
      181 ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      241 ctcagttcac ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      301 tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      421 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      481 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga
      541 tgaggctgaa gctgaaggac ggcggccact acacctccga ggtcaagacc acctacaagg
      601 ccaagaagcc cgtgcagctg cccggcgcct acatcgtcgg catcaagttg gacatcacct
      661 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      721 ccggcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
      781 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      841 gccataccac atttgtagag gtt
//
