LOCUS       sequence-91823-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 472, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MiCy-N1
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..804
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gtcttattca
      121 aagcaaggca tcgcacaaga aatgcggacg aaataccgta tggaaggcag tgtcaatggc
      181 catgagttca cgatcgaagg tgtaggaact ggaaaccctt acgaagggaa acagatgtcc
      241 gaattagtga tcatcaagcc taagggaaaa ccccttccat tctcctttga catactgtca
      301 acagcctttc aatatggaaa cagatgcttc acaaagtacc ctgccgacat gcctgactat
      361 ttcaagcaag cattcccaga tggaatgtca tatgaaaggt catttctatt tgaggatgga
      421 ggagttgcta cagccagctg gagcattcgt ctcgaaggaa attgcttcat ccacaattcc
      481 atctatcatg gcgtaaactt tcccgctgat ggacccgtaa tgaagaagca gacaattggc
      541 tgggataagt ccttcgaaaa aatgagtgtg gctaaagagg tgctaagagg tgatgtgact
      601 cagtttcttc tgctcgaagg aggtggttac cagagatgcc ggtttcactc cacttacaaa
      661 acggagaagc cagtcgcaat gcccccgagt catgtcgtag aacatcaaat tgtgaggacc
      721 gaccttggcc aaactgcaaa aggcttcaag gtcaagctgg aagaacatgc tgaggctcat
      781 gttaaccctt tgaaggttaa ataagcggcc gcgactctag atcataatca gccataccac
      841 atttgtagag gttttacttg ctttaaa
//
