LOCUS       sequence-91824-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LexA-ER-B42 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(93..342)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     308..326
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     369..385
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     370..386
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(419..655)
                     /codon_start=1
                     /product="yeast transcriptional activator cretaed from E. 
                     coli genomic DNA fragments (Ma and Ptashne, 1987)"
                     /label=B42 transcriptional activator
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
ORIGIN
        1 ggccgaagct gggtaccaag cagacgctac taaggaaaac aacaacggaa gcgatgatga
       61 gagcgacgat gagagtgtaa actgcgaacc ttgcaaatta aagccttcga gcgtcccaaa
      121 accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgttt gtacagaaaa
      181 aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat taaaaaaaat
      241 aaatagggac ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg
      301 gaggagggcg tgaatgtaag cgtgacataa ctaattacat gatatagaca aaggaaaagg
      361 ggcctgtctc gaggtcgacg gtatcgataa gctttaaacg cgtttaaagc ttggaggcat
      421 ctccactcag caagaggctg gtatcgttaa catccgcttc aatttcatga aaaccattcg
      481 atttcagcca gtcaggctgg atcggcggcc cggcgtgaag aatggttttc ggcgtcatgc
      541 ccggcaccac gttaatcgcc tgatccgcca tttccatcgg catctcctgt ccggtgcgca
      601 ggtagtcgat aaactgctca atgatggcat tgcactcctc gatgtcttta ttgataccgc
      661 ccgcggtgct gttatcgttg cccggcagat cctgcatgat atcgactgtg gcagggaaac
      721 cctctgcctc ccccgtgatg taatactttt gcaaggaatg cgatgaagta gagcccgcag
      781 tggccaagtg gctttggtcc gtctcctcca cggatgcccc tccacggcta gtgggcgcat
      841 gtaggcggtg ggcgtccagc atctccagca gcaggtcata gaggggcacc acgttcttgc
      901 acttcatgct gtacagatgc tccatgcctt tgttactcat gtgcctgatg tgggagagga
      961 tgaggagga
//
