LOCUS       sequence-91825-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..799
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagcg agctgattaa
      121 ggagaacatg cccatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg
      181 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtcgt
      241 cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag
      301 cagaaccttc atcaagcacc ctccgggcat ccccgacttc tttaagcagt ccttccctga
      361 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
      421 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aaggttagag gggtgaactt
      481 cccagccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac
      541 gatgtacccc gctgacggcg gcctggaagg cgcatgtgac atggccctga agctcgtggg
      601 cgggggccac ctgatctgca accttgagac cacatacaga tccaagaaac ccgctacgaa
      661 cctcaagatg cccggcgtct acaacgtgga ccacagactg gaaagaatca aggaggccga
      721 cgatgagacc tacgtcgagc agcacgaggt ggctgtggcc agatactcta ctggtggcgc
      781 tggtgatgga ggtaaatgag cggccgcgac tctagatcat aatcagccat accacatt
//
