LOCUS       sequence-91826-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-N1
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..804
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcgagctg
      121 attaaggaga acatgcccat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
      181 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
      241 gtcgtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
      301 ggcagcagaa ccttcatcaa gcaccctccg ggcatccccg acttctttaa gcagtccttc
      361 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
      421 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaaggt tagaggggtg
      481 aacttcccag ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggcctccacc
      541 gagacgatgt accccgctga cggcggcctg gaaggcgcat gtgacatggc cctgaagctc
      601 gtgggcgggg gccacctgat ctgcaacctt gagaccacat acagatccaa gaaacccgct
      661 acgaacctca agatgcccgg cgtctacaac gtggaccaca gactggaaag aatcaaggag
      721 gccgacgatg agacctacgt cgagcagcac gaggtggctg tggccagata ctctactggt
      781 ggcgctggtg atggaggtaa atgagcggcc gcgactctag atcataatca gccataccac
      841 atttgtagag gtt
//
