LOCUS       sequence-91830-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - BirA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-birA-tagRFP
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcgtaatac gactcactat agggcgaatt gggtaccaaa tagtaggaat tacccacctg
       61 tacaagtgct gaaaacttgg atgaataagc ccgtttgcca ttttctactg ctattttaaa
      121 tcttttctgt atttgtctgc atttgtcttt acccttgaat aaatgtttta gttgtttttt
      181 ttcaattatg actgtgttta acagacatta ttacaataat atgtaatagt agtacacact
      241 attattatgt aatagtactt gttgactgta tttgagaact ggaccgagtg agtgttacgt
      301 cacccattca aaatgactta cttctggctc caacaaaatg aagttaattc agttgccatt
      361 tttcactgta tggacatcgc cgtgttggag ctagacgttg ccaataagca agaaagagcc
      421 gagatgcgtc gagtctgagt caccgttcct atggcaaccc ctctaaccaa tcagaagtaa
      481 gcttgttgga agtccacagc ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt
      541 tttgaacgtt ggatgtgaga gcacatactt ttattaaggc atctggttgg tcagtttata
      601 atatcaacaa cttgggctac agaaaagaaa agttattaca gaaattatga ttaacaagta
      661 catgttaaat aaagatttta atatgaatgc caccactgga gcattcatgc catttggagc
      721 ttcttcctgt ttggatcact agaaggagga ggtcactcat tacagttctc atatacagtc
      781 gttggttggt tggttggttg gtagattgat tgattgattg attgattgat tgattgattg
      841 attgattgat tgattgattg attgattgat tgattgattg gtagtcaaaa taagaaataa
      901 tttccacaga ttcattacag aaatgattaa atgcatacat aaaaaactgg gggg
//
