LOCUS       sequence-91832-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - BirA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-birA-tagRFP
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..754)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     regulatory      751..760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgtctggatc atcatcgatt tacttgtaca gctcgtccat gccattaagt ttgtgcccca
       61 gtttgctagg gaggtcgcag tatctggcca cagccacctc gtgctgctcg acgtaggtct
      121 ctttgtcggc ctccttgatt ctttccagtc tgtggtccac atagtagacg ccgggcatct
      181 tgaggttctt agcgggtttc ttggatctgt atgtggtctt gaagttgcag atcaggtggc
      241 ccccgcccac gagcttcagg gccatgtcgg ttctgccttc caggccgccg tcagcggggt
      301 acagcatctc ggtgttggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg
      361 atgggaagtt cacccctctg atcttgacgt tgtagatgag gcagccgtcc tggaggctgg
      421 tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgactctc tcccatgtga
      481 agccctcagg gaaggactgc ttaaagaaat cggggatgcc ctgggtgtgg ttgatgaagg
      541 ttctgctgcc gtacatgaag ctggtagcca ggatgtcgaa ggcgaagggg agagggccgc
      601 cctcgaccac cttgattctc atggtctggg tgccctcgta gggcttgcct tcgccctcgg
      661 atgtgcactt gaagtggtgg ttgttcacgg tgccctccat gtacagcttc atgtgcatgt
      721 tctccttaat cagctcttcg cccttagaca ccatggtggc aagctttttt tctgcactac
      781 gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca agtaataaag
      841 ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca atgataagtt
      901 tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa ggtgccaatc
      961 cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg gccgccaacg
     1021 tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga ttaac
//
