LOCUS       sequence-91833-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - BirA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-birA-tagRFP
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(29..991)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(29..991)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 cgcccttaga caccatggtg gcaagctttt tttctgcact acgcagggat atttcaccgc
       61 ccatccaggg ttttattatt ccatcctgct caagtaataa agccccctgt ttgtctattc
      121 cgcgtgaaat gccaaatatt tctttatcac caatgataag tttcactggg cgattaataa
      181 aattatccag cttttcccag cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt
      241 ccaacgcagc acgtaattca cgtattagca tggccgccaa cgtattacga tcgagattga
      301 tccccgcttc ctgcagcgtg atccacccct gattaacgac actctcttca acacggcgca
      361 ttgccatgtt gatcccggct ccaatgacta tttgcgccgc atcgccagtt ttgccagtca
      421 gctccaccag aatgcctgcc agcttgcgat cctgcagata gaggtcatta ggccatttaa
      481 cacgaacttt atctgcaccc agcttgcgta atacttccgc catcacgata ccgataacca
      541 gacttaaacc aatcgccgcc gccgggcctt gttccagacg ccagaacatc gacaaatata
      601 agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg accacggcca gcctgctggt
      661 attctgcaat gcaagcatcg cccgatttaa gctctccgat acgatcaaga aggtactgat
      721 tcgtggagtc aatcacaggc agcacggcta cactaccgcc atccagctga cccaatatct
      781 gtttagcatt aagtaactgg ataggctcag gcaggctgta tcctttaccc ggaacggtaa
      841 agacatcaac gccccagtca cgcagtgtct gaatgtgttt attaatagcc gcccggctca
      901 ttcccagcgt ttcacccaac tgctcgccag agtgaaattc accgttcgct aacagggcaa
      961 tcaatttcag tggcacggtg ttatccttca tggtggcgga tccgaattcg gccggccagg
     1021 cctttaatta actcgagggc ccatctggcc tg
//
