LOCUS       sequence-91839-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(78..89,90..252)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             671..715
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(767..788)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
       61 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      121 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      181 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      241 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      301 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      361 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      421 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
      481 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
      541 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
      601 acacaggcca gatgggccct cgagttaatt aaaggcctgg ccggccgaat tcggatccgc
      661 caccatgtca ggactgaacg atatcttcga ggctcagaaa atcgagtggc acgagggcgc
      721 gccagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      781 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      841 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      901 cctcgtgacc accctgacct a
//
