LOCUS       sequence-91844-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             610..618
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(735..756)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcgatctagt agaggcgctg tggttttccc atggtgctct tgatgtaaag agctctgacg
       61 ttttgccagt tcttcttcag cagagacacc aggaagttga cagccaggtg gatgttgtag
      121 accagctctt cctcactcat cttcacatga cccacggcca cagccagaca cagaaccttc
      181 ttcatctgga atttgatggt ggatttcacc tcatccacct tggtgcccag gttctcatta
      241 tgggtgagca gagagggaaa ctttcctgcc ttgttgaggc cagggcccag gatacgagga
      301 atctgcttga tcagagactc agaggccagg aaagcatcgt acttctttgc cagcttcttg
      361 accagcttct tgttcttatt gagcttctta agagcctcaa tgtccatgtg tggcagttca
      421 gcggcctttg cttcatcaca atgctgctgg tctccaagaa cgcagacaga gaacttgggc
      481 cgcggggtgg tcttcagcct gacagtgcct gagaaacgct tgtccttctg agggtcgtag
      541 ttcttcaagc tgatctggag ttccacggtt tcaagaaact ttctcttctt gcgacgggaa
      601 ccggcctgga cctccttaac ggcctcgtac aacgtgtctc tcgagacctt gctgaattcg
      661 aagcttgagc tcgagatctg agtccggccg gacttgtaca gctcgtccat gccgagagtg
      721 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      781 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      841 ccgatggggg tgttctgctg gtagtggccg gcgagctgca cgctgccgtc ctcgatgttg
      901 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      961 ttgtggctgt tgtagttgta ctccagcttg tgccccagga
//
