LOCUS       sequence-91846-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses dominant negative human PKCi tagged with V5 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Wald FA, Oriolo AS, Mashukova A, Fregien NL, Langshaw AH, Salas PJ
  TITLE     Atypical protein kinase C (iota) activates ezrin in the apical 
            domain of intestinal epithelial cells.
  JOURNAL   J Cell Sci. 2008 Mar 1;121(Pt 5):644-54. doi: 10.1242/jcs.016246. 
            Epub 2008 Feb 12.
  PUBMED    18270268
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2008-03-1"; volume: "121(Pt 5):644-54. doi"; pages: " 
            10.1242/jcs.016246. Epub 2008 Feb 12"
COMMENT     Sequence Label: pcDNATM3.1/V5-His TOPO-PKCi-K274W
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatccag tacccttcac catgtcccac
      121 acggtcgcag gcggcggcag cggggaccat tcccaccagg tccgggtgaa agcctactac
      181 cgcggggata tcatgataac acattttgaa ccttccatct cctttgaggg cctttgcaat
      241 gaggttcgag acatgtgttc ttttgacaac gaacagctct tcaccatgaa atggatagat
      301 gaggaaggag acccgtgtac agtatcatct cagttggagt tagaagaagc ctttagactt
      361 tatgagctaa acaaggattc tgaactcttg attcatgtgt tcccttgtgt accagaacgt
      421 cctgggatgc cttgtccagg agaagataaa tccatctacc gtagaggtga acgccgctgg
      481 agaaagcttt attgtgccaa tggccacact ttccaagcca agcgtttcaa caggcgtgct
      541 cactgtgcca tctgcacaga ccgaatatgg ggacttggac gccaaggata taagtgcatc
      601 aactgcaaac tcttggttca taagaagtgc cataaactcg tcacaattga atgtgggcgg
      661 cattctttgc cacaggaacc agtgatgccc atggatcagt catccatgca ttctgaccat
      721 gcacagacag taattccata taatccttca agtcatgaga gtttggatca agttggtgaa
      781 gaaaaagagg caatgaacac cagggaaagt ggcaaagctt catccagtct aggtcttcag
      841 gattttgatt tgctccgggt aataggaaga ggaagttatg ccaaagtact gttggttcga
      901 ttaaaaaaaa cagatcgtat tt
//
