LOCUS       sequence-91849-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(121..633,634..636))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     314..333
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     573..594
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(646..690)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
       61 ggggccgtcg ccgatggggg tgttctgctg gtagtggccg gcgagctgca cgctgccgtc
      121 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      181 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      241 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      301 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      361 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      421 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      481 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      541 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      601 caccaccccg gtgaacagct cctcgccctt gctcactggc gcgccctcgt gccactcgat
      661 tttctgagcc tcgaagatat cgttcagtcc tgacatggtg gcggatccga attcggccgg
      721 ccaggccttt aattaactcg agggcccatc tggcctgtgt ttcagacacc agggagtctc
      781 tgctcacgtt tcctgctatt tgcagcctct ctatcaagac taatacacct cttcccgcat
      841 cggctgcctg tgagaggctt ttcagcactg caggattgct tttcagcccc aaaagagcta
      901 ggcttgacac taacaatttt gagaatcagc ttctactgaa gttaaatctg aggttttaca
      961 actttgagta gcgtgtactg gcattagatt gtctgtctta tagtttgata attaaataca
     1021 aacagttcta aagcagga
//
