LOCUS       sequence-91853-e        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..42
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     79..95
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     79..95
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(129..140,141..328)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    767..901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(804..823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     858..877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
       61 caccgcggtg gcggccgctc tagaactagt ggatctgctg ggcttgctga aggtaggggg
      121 tcaagaacca gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt
      181 atttttgggg atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact
      241 taattacatt tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca
      301 aaaagtactt attttttgga gatcacttca ttctattttc ccttgctatt accaaaccaa
      361 ttgaattgcg ctgatgccca gtttaattta aatgttattt attctgccta tgaaaatcgt
      421 tttcacatta tatgaaattg gtcagacatg ttcattggtc ctttggaagt gacgtcatgt
      481 cacatctatt accacaatgc acagcacctt gacctggaaa ttagggaaat tataacagtc
      541 aatcagtgga agaaaatgga ggaagtatgt gattcatcag cagctgcgag cagcacagtc
      601 caaaatcagc cacaggatca agagcacccg tggccgtatc ttcgcagatc tgatctagag
      661 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      721 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
      901 catcatcgat ctagtagagg cgctgtggtt ttcccatggt gctcttgatg taaagagctc
      961 tgacgttttg ccagttcttc ttcagcagag acaccaggaa gttgacagcc aggtggatgt
     1021 tgtagaccag ctcttcctca ctcatcttca catgacccac ggccacagcc agacacagaa
     1081 ccttcttcat ctggaatttg atg
//
