LOCUS       sequence-91857-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - BirA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-birA-EGFP
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accaaatagt aggaattacc cacctgtaca
       61 agtgctgaaa acttggatga ataagcccgt ttgccatttt ctactgctat tttaaatctt
      121 ttctgtattt gtctgcattt gtctttaccc ttgaataaat gttttagttg ttttttttca
      181 attatgactg tgtttaacag acattattac aataatatgt aatagtagta cacactatta
      241 ttatgtaata gtacttgttg actgtatttg agaactggac cgagtgagtg ttacgtcacc
      301 cattcaaaat gacttacttc tggctccaac aaaatgaagt taattcagtt gccatttttc
      361 actgtatgga catcgccgtg ttggagctag acgttgccaa taagcaagaa agagccgaga
      421 tgcgtcgagt ctgagtcacc gttcctatgg caacccctct aaccaatcag aagtaagctt
      481 gttggaagtc cacagcctac cacttgaaag cgggctgcac aaaatctgtc aaacgttttg
      541 aacgttggat gtgagagcac atacttttat taaggcatct ggttggtcag tttataatat
      601 caacaacttg ggctacagaa aagaaaagtt attacagaaa ttatgattaa caagtacatg
      661 ttaaataaag attttaatat gaatgccacc actggagcat tcatgccatt tggagcttct
      721 tcctgtttgg atcactagaa ggaggaggtc actcattaca gttctcatat acagtcgttg
      781 gttggttggt tggttggtag attgattgat tgattgattg attgattgat tgattgattg
      841 attgattgat tgattgattg attgattgat tgattggtag tcaaaataag aaataatttc
      901 cacagattca ttacagaaat gattaa
//
