LOCUS       sequence-91867-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the pH sensor ratiometric pHluorin in the mitochondria of 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV064: 1xCox8 - ratiometric pHluorin in fck
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             418..492
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(553..581)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tatagttgga ggtgggggag gtaggaagag cgatgatcac ttgtggacta agtttgttcg
       61 catccccttc tccaaccccc tcagtacatc accctggggg aacagggtcc acttgctcct
      121 gggcccacac agtcctgcag tattgtgtat ataaggccag ggcaaagagg agcaggtttt
      181 aaagtgaaag gcaggcaggt gttggggagg cagttaccgg ggcaacggga acagggcgtt
      241 tcggaggtgg ttgccatggg gacctggatg ctgacgaagg ctcgcgaggc tgtgagcagc
      301 cacagtgccc tgctcagaag ccccaagctc gtcagtcaag ccggttctcc gtttgcactc
      361 aggagcacgg gcaggcgagt ggcccctagt tctgggggca gcgggggatc cgccaccatg
      421 tccgtcctga cgccgctgct gctgcggggc ttgacaggct cggcccggcg gctcccagtg
      481 ccgcgcgcca agatccattc gttgggtgac ccgccagtcg ctagcagtaa aggagaagaa
      541 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      601 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaa
//
