LOCUS       sequence-91868-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the calcium sensor GCaMP5G in the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV073: 1xCox8 - GCaMP5G in fubi
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             418..492
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             532..549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             553..585
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(589..597,598..612)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             640..696
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 tatagttgga ggtgggggag gtaggaagag cgatgatcac ttgtggacta agtttgttcg
       61 catccccttc tccaaccccc tcagtacatc accctggggg aacagggtcc acttgctcct
      121 gggcccacac agtcctgcag tattgtgtat ataaggccag ggcaaagagg agcaggtttt
      181 aaagtgaaag gcaggcaggt gttggggagg cagttaccgg ggcaacggga acagggcgtt
      241 tcggaggtgg ttgccatggg gacctggatg ctgacgaagg ctcgcgaggc tgtgagcagc
      301 cacagtgccc tgctcagaag ccccaagctc gtcagtcaag ccggttctcc gtttgcactc
      361 aggagcacgg gcaggcgagt ggcccctagt tctgggggca gcgggggatc cgccaccatg
      421 tccgtcctga cgccgctgct gctgcggggc ttgacaggct cggcccggcg gctcccagtg
      481 ccgcgcgcca agatccattc gttgggtgac ccgccagtcg ctagcggttc tcatcatcat
      541 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      601 gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca
      661 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc
      721 gacaagcaga agaacggcat caaggcgaac ttc
//
