LOCUS       sequence-91869-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses channelrhodopsin-2 with the C128S (step function opsin) 
            mutation fused to eGFP under the ubiquitin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV201: ChR2(C128S)-eGFP in fubi
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      147..156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
       61 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
      121 ggctgcaggt cgactctaga ggatccgcca ccatggacta tggcggcgct ttgtctgccg
      181 tcggacgcga acttttgttc gttactaatc ctgtggtggt gaacgggtcc gtcctggtcc
      241 ctgaggatca atgttactgt gccggatgga ttgaatctcg cggcacgaac ggcgctcaga
      301 ccgcgtcaaa tgtcctgcag tggcttgcag caggattcag cattttgctg ctgatgttct
      361 atgcctacca aacctggaaa tctacatgcg gctgggagga gatctatgtg tgcgccattg
      421 aaatggttaa ggtgattctc gagttctttt ttgagtttaa gaatccctct atgctctacc
      481 ttgccacagg acaccgggtg cagtggctgc gctatgcaga gtggctgctc actagtcctg
      541 tcatccttat ccacctgagc aacctcaccg gcctgagcaa cgactacagc aggagaacca
      601 tgggactcct tgtctcagac atcgggacta tcgtgtgggg ggctaccagc gccatggcaa
      661 ccggctatgt taaagtcatc ttcttttgtc ttggattgtg ctatg
//
