LOCUS       sequence-91878-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  self-inactivating lentiviral plasmid for co-expression of your gene 
            of interest and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z
  TITLE     Lentiviral transduction of mammary stem cells for analysis of gene 
            function during development and cancer.
  JOURNAL   Cell Stem Cell. 2008 Jan 10. 2(1):90-102.
  PUBMED    18371425
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Jan 10. 2(1):90-102."
COMMENT     Sequence Label: pHIV-EGFP
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(56..630,631..633,634..642)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(223..240)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     450..469
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(688..709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggtgtcgtga gcggccgctg agttaactat tctagagtac ccgggctagg atccgcccct
       61 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
      121 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
      181 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
      241 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
      301 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
      361 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
      421 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
      481 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
      541 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
      601 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
      661 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      721 aagttcagcg tgtccggcga gggcgagggc gatgcc
//
