LOCUS       sequence-91880-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: E[beta]P
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             171..206
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ggtgtcgtga ggaattagct tggtactaat acgactcact atagggagac ccaagctggc
       61 taggtaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tcccgggata
      121 acaccatggc tactcaagct gacctgatgg agttggacat ggccatggag ccggacagaa
      181 aagctgctgt cagccactgg cagcagcagt cttacttgga tgctggaatc catgctggtg
      241 ccaccgccac agctcctgcc ctgagtggca agggcaaccc tgaggaagaa gatgttgaca
      301 cctcccaagt cctttatgaa tgggagcaag gcttttccca gtccttcacg caagagcaag
      361 tagctgatat tgacgggcag tatgcaatga ctagggctca gagggtccga gctgccatgt
      421 tccctgagac gctagatgag ggcatgcaga tcccatccac gcagtttgac gctgctcatc
      481 ccactaatgt ccagcgcttg gctgaaccat cacagatgtt gaaacatgca gttgtcaatt
      541 tgattaacta tcaggatgac gcggaacttg ccacacgtgc aattcctgag ctgacaaaac
      601 tgctaaacga tgaggaccag gtggtagtta ataaagctgc tgttatggtc catcagcttt
      661 ccaaaaagga agcttccaga catgccatca tgcgctcccc tcagatggtg tctgccattg
      721 tacgcaccat gcagaataca aatgatgtag agacagctcg ttgtactgct gggactctgc
      781 acaacctttc tcaccaccgc gagggcttgc tggccatctt taagtctggt ggcatcccag
      841 cgctggtgaa aatgcttggg tcaccagtgg attctgtact gttctacgcc atcacgac
//
