LOCUS       sequence-91888-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the forkhead binding site and the 
            C-terminal domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES11L-dFC
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      121 ctggaattcg ccatggacta caaagacgat gacgacaagg gcatgggtcc agtcatgcct
      181 cccagtaaga agccagaaag ctcaggaatt agtgtctcca gtggactgag tcagtgttac
      241 gggggcagcg gtttctccaa ggcccttcag gaagacgatg acctcgactt ttctctgcct
      301 gacatccgat tagaagaggg ggccatggaa gatgaagagc tgaccaacct gaactggctg
      361 cacgagagca agaacttgct gaagagcttt ggggagtcgg tcctcaggag tgtcagcccc
      421 gtccaggacc tggacgatga caccccccca tcccctgccc actctgacat gccctacgat
      481 gccaggcagt aactcgagca tgcatctaga gggccgataa aataaaagat tttatttagt
      541 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
      601 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
      661 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aa
//
