LOCUS       sequence-91890-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the C-terminal and NLS domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1L-dC
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             133..156
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      121 gaattcgcca tggactacaa agacgatgac gacaagggca tgggtccagt catgcctccc
      181 agtaagaagc cagaaagctc aggaattagt gtctccagtg gactgagtca gtgttacggg
      241 ggcagcggtt tctccaaggc ccttcaggaa gacgatgacc tcgacttttc tctgcctgac
      301 atccgattag aagagggggc catggaagat gaagagctga ccaacctgaa ctggctgcac
      361 gagagcaaga acttgctgaa gagctttggg gagtcggtcc tcaggagtgt cagccccgtc
      421 caggacctgg acgatgacac ccccccatcc cctgcccact ctgacatgcc ctacgatgcc
      481 aggcagaacc ccaactgcaa acccccctac tccttcagct gcctcatatt tatggccatc
      541 gaggactctc caaccaagcg cctgccagtg aaggatatct acaactggat cttggaacat
      601 tttccgtatt ttgcaaatgc acctactggg tggaaaaact cagtgagaca caatttatca
      661 ttgaataa
//
