LOCUS       sequence-91891-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the C-terminal, with remaining NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1L-dC-NLS
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             128..151
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg tgctggaatt
      121 cgccatggac tacaaagacg atgacgacaa gggcatgggt ccagtcatgc ctcccagtaa
      181 gaagccagaa agctcaggaa ttagtgtctc cagtggactg agtcagtgtt acgggggcag
      241 cggtttctcc aaggcccttc aggaagacga tgacctcgac ttttctctgc ctgacatccg
      301 attagaagag ggggccatgg aagatgaaga gctgaccaac ctgaactggc tgcacgagag
      361 caagaacttg ctgaagagct ttggggagtc ggtcctcagg agtgtcagcc ccgtccagga
      421 cctggacgat gacacccccc catcccctgc ccactctgac atgccctacg atgccaggca
      481 gaaccccaac tgcaaacccc cctactcctt cagctgcctc atatttatgg ccatcgagga
      541 ctctccaacc aagcgcctgc cagtgaagga tatctacaac tggatcttgg a
//
