LOCUS       sequence-91895-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector (AmpR, high copy, blue/white selection, M13 
            IR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Vieira J, Messing J
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins.
  JOURNAL   Gene. 1991 Apr;100:189-94.
  PUBMED    1905257
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1991-04"; volume: "100"; pages: "189-94"
COMMENT     Sequence Label: pUC21
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..123
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     107..123
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(137..156)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(138..156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(163..180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(163..179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(172..194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(479..498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     611..632
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggcctcgag atctatcgat gcatgccatg gtacccggga gctcgaattc gaagcttctg
       61 cagacgcgtc gacgtcatat ggatccgata tcgccgtggc ggccgctcta gaactagtgg
      121 atcgatcccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      181 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      241 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      301 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      361 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
      421 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
      481 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      541 aatcggggtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      601 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
      661 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
      721 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttaca
//
