LOCUS       sequence-91896-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector (KanR, high copy, blue/white selection, M13 
            IR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Vieira J, Messing J
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins.
  JOURNAL   Gene. 1991 Apr;100:189-94.
  PUBMED    1905257
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1991-04"; volume: "100"; pages: "189-94"
COMMENT     Sequence Label: pUK21
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     114..130
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     114..130
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(144..163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(145..163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(170..187)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(170..186)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(179..201)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(486..505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     618..639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tagtccgagg cctcgagatc tatcgatgca tgccatggta cccgggagct cgaattcgaa
       61 gcttctgcag acgcgtcgac gtcatatgga tccgatatcg ccgtggcggc cgctctagaa
      121 ctagtggatc gatccccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
      181 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      241 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      301 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      361 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
      421 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
      481 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      541 agctctaaat cggggtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      601 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
      661 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
      721 gctgatttaa caaaaattta acgcgaattt taacaaatat taacgtttac aat
//
