LOCUS       sequence-91898-e         538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red fluorescent indicator for calcium signaling in the endoplasmic 
            reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV R-CEPIA1er
FEATURES             Location/Qualifiers
     source          1..538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     join(54..98,181..192)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             join(54..99,182..192)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             229..285
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 actagagaac ccactgctta ctggcacgtg gaaattaata cgacgtggcc accatgggat
       61 ggagctgtat catcctcttc ttggtagcaa cagctacagg taaggggtta acagtagcag
      121 gcttgaggtc tggacatata tatgggtgac aatgacatcc actttgcctt tctctccaca
      181 ggcgcgcact cccaggtcca actgcaggga tccatggtcg actcttcacg tcgtaagtgg
      241 aataaggcag gtcacgcagt cagagctata ggtcggctga gctcacccgt ggtttccgag
      301 cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaaggggct gaggctgaag
      361 gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
      421 ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac
      481 accatcgtgg aacagtgcga acgcgccgag ggccgccact ccaccggcgg catggacg
//
