LOCUS       sequence-91908-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red fluorescent indicator for calcium signaling in the endoplasmic 
            reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV R-CEPIA1er
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..59)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(69..509)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKDLGTVLRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPDFLTMMARKMKDTDSEEEIREAFRVWDKDGNGYISAADL
                     RHVMTNLGEKLTDEEVDEMIREADIDGEGQVNYEEFVQMMTAK"
     primer_bind     complement(533..552)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agctcgtcct tctcgcttgc ggccccattc agatcctctt ctgagatgag tttttgttct
       61 gcggccgcct tcgctgtcat catttgtaca aactcttcgt agtttacctg accttctcca
      121 tcgatatctg cttccctgat catttcatca acctcttcat ctgttaactt ctctccaagg
      181 tttgtcatca cgtggcgaag gtctgctgca gagatgtagc cattgccatc cttatcccac
      241 acacggaacg cttcgcgaat ttcttcttca ctgtctgtgt ctttcatttt tcttgccatc
      301 attgtcagga aatcagggaa gtcgattgtg ccattaccgt cggcatctac ttcattgatc
      361 atgtcctgga gctctgcttc tgtggggttc tgccccagag accgcagcac cgtccccaga
      421 tccttggttg ttattgtccc atccccgtcc ttgtcaaata gggagaaagc ctctttaaat
      481 tctgcgatct gctcttcagt cagttggtct cgagtagcct cccagcccat ggtcttcttc
      541 tgcattacgg ggccgtcggg ggggaagttg gtgccgcgca gcttcacctt gtagatgaat
      601 acgccgtcct gcagggagga gtcctggtta acgtgaataa tgccgccgtc ctcgaagttc
      661 atcacgcgct cccacct
//
