LOCUS       sequence-91913-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the forkhead binding site and the 
            C-terminal domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES11L-dFC
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(370..393)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(461..480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(462..480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(525..728)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(554..574)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cttttatttt atcggccctc tagatgcatg ctcgagttac tgcctggcat cgtagggcat
       61 gtcagagtgg gcaggggatg ggggggtgtc atcgtccagg tcctggacgg ggctgacact
      121 cctgaggacc gactccccaa agctcttcag caagttcttg ctctcgtgca gccagttcag
      181 gttggtcagc tcttcatctt ccatggcccc ctcttctaat cggatgtcag gcagagaaaa
      241 gtcgaggtca tcgtcttcct gaagggcctt ggagaaaccg ctgcccccgt aacactgact
      301 cagtccactg gagacactaa ttcctgagct ttctggcttc ttactgggag gcatgactgg
      361 acccatgccc ttgtcgtcat cgtctttgta gtccatggcg aattccagca cactggcggc
      421 cgttactagt ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta
      481 atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag
      541 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
      601 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
      661 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
      721 cgcatcacca tggtaatagc gatga
//
