LOCUS       sequence-91914-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the forkhead binding site and C-terminal 
            domain, with remaining NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1LdFC-NLS
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..66)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(397..420)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(488..507)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(489..507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(552..755)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(581..601)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 taaaatcttt tattttatcg gccctctaga tgcatgctcg agttatacct tacgcttctt
       61 ctttggctgc ctggcatcgt agggcatgtc agagtgggca ggggatgggg gggtgtcatc
      121 gtccaggtcc tggacggggc tgacactcct gaggaccgac tccccaaagc tcttcagcaa
      181 gttcttgctc tcgtgcagcc agttcaggtt ggtcagctct tcatcttcca tggccccctc
      241 ttctaatcgg atgtcaggca gagaaaagtc gaggtcatcg tcttcctgaa gggccttgga
      301 gaaaccgctg cccccgtaac actgactcag tccactggag acactaattc ctgagctttc
      361 tggcttctta ctgggaggca tgactggacc catgcccttg tcgtcatcgt ctttgtagtc
      421 catggcgaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccaagct
      481 tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta
      541 gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      601 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      661 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
      721 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
      781 taaatgtact gccaagta
//
