LOCUS       sequence-91921-e         449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant encoding the DNA binding and NLS domains only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1L-DBD-NLS
FEATURES             Location/Qualifiers
     source          1..449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..26)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(318..341)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(409..428)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(410..428)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agttatacct tacgcttctt ctttggcttt ttcaaagcct gaattagatt ttgtctatac
       61 tctgggtcta tgcaccacaa cgaccctttc ccaatactct gactcctctc tttgtccact
      121 ttcttaaaac acttattcaa tgataaattg tgtctcactg agtttttcca cccagtaggt
      181 gcatttgcaa aatacggaaa atgttccaag atccagttgt agatatcctt cactggcagg
      241 cgcttggttg gagagtcctc gatggccata aatatgaggc agctgaagga gtaggggggt
      301 ttgcagttgg ggttgccctt gtcgtcatcg tctttgtagt ccatggcgaa ttccagcaca
      361 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttgggtctcc ctatagtgag
      421 tcgtattaat ttcgataagc cagtaagca
//
