LOCUS       sequence-91930-e         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent indicator for calcium imaging in the mitochondria 
            (moderately low affinity variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV CEPIA3mt
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    98..322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     311..328
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(364..393)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac
       61 cccctagagc cccagctggt tctttccgcc tcagaagcca tagagcccgg gccatcccca
      121 gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc
      181 agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg
      241 acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat
      301 ggctggcaac tagaaggcac agtcgaggct gatcagcgag cttctagact atgcggcccc
      361 attcagatcc tcttctgaga tgagtttttg ttctgcggcc gccttcgctg tcatcatttg
      421 tacaaactct tcgtagttta cctgaccatc cccatcgatg tctgcttccc tgatcatttc
      481 atc
//
