LOCUS       sequence-91950-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used for anti-mouse Patched antibody production (for affinity 
            purification).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Rohatgi R, Milenkovic L, Scott MP
  TITLE     Patched1 regulates hedgehog signaling at the primary cilium.
  JOURNAL   Science. 2007 Jul 20;317(5836):372-6.
  PUBMED    17641202
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-07-20"; volume: "317(5836)"; pages: "372-6"
COMMENT     Sequence Label: pMal mPtch-Cterm
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..31
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             32..52
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 caataacaac aacctcggga tcgagggaag ggagaatctt tattttcagg gcgcggcagc
       61 catgggatcc ggccggccgg attcctccga ctcggagtac agctctcaga ccacggtgtc
      121 tggcatcagt gaggagctca ggcaatacga agcacagcag ggtgccggag gccctgccca
      181 ccaagtgatt gtggaagcca cagaaaaccc tgtctttgcc cggtccactg tggtccatcc
      241 ggactccaga catcagcctc ccttgacccc tcggcaacag ccccacctgg actctggctc
      301 cttgtcccct ggacggcaag gccagcagcc tcgaagggat ccccctagag aaggcttgcg
      361 gccacccccc tacagaccgc gcagagacgc ttttgaaatt tctactgaag ggcattctgg
      421 ccctagcaat agggaccgct cagggccccg tggggcccgt tctcacaacc ctcggaaccc
      481 aacgtccacc gccatgggca gctctgtgcc cagctactgc cagcccatca ccactgtgac
      541 ggcttctgct tcggtgactg ttgctgtgca tcccccgcct ggacctgggc gcaacccccg
      601 aggggggccc tgtccaggct atga
//
