LOCUS       sequence-91952-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of drosophila YFP Rab18 dominant negative mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Zhang J, Fonovic M, Suyama K, Bogyo M, Scott MP
  TITLE     Rab35 controls actin bundling by recruiting fascin as an effector 
            protein.
  JOURNAL   Science. 2009 Sep 4;325(5945):1250-4. doi: 10.1126/science.1174921.
  PUBMED    19729655
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-09-4- 4"; volume: "325"; issue: 
            "5945"; pages: "1250-4"
COMMENT     Sequence Label: pUAST YFP Rab18 DN
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(272..292)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          422..487
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    580..604
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
       61 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
      121 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
      181 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
      241 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag
      301 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca
      361 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa
      421 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
      481 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
      541 agtaaggttc cttcacaaag atcctctaga ggatcatcaa ccactttgta caagaaagct
      601 gggtttcaac agtaacacgt agacgcgctt gcctccagat cccgatcgct ggcgatgtcc
      661 agaccggcgg aggcattgcc attgttgaag tattcggagg agacgatctt ctccaccaca
      721 tctttgaaca catcgctgac gaactgatcg catt
//
