LOCUS       sequence-91953-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Hitchcock AL, Krebber H, Frietze S, Lin A, Latterich M, Silver PA
  TITLE     The conserved npl4 protein complex mediates proteasome-dependent 
            membrane-bound transcription factor activation.
  JOURNAL   Mol Biol Cell 2001 Oct;12(10):3226-41.
  PUBMED    11598205
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2001-10"; volume: "12(10)"; pages: "3226-41"
COMMENT     Sequence Label: pPS2351
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..715
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(32..60)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     655..675
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(717..733)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(717..733)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatgagtaaa ggagaagaac tcttcactgg agttgtccca attcttgttg aattagatgg
       61 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      121 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      181 tgtcactact ttctcttatg gtgttcaatg cttttcaaga tacccggatc atatgaaacg
      241 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      301 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      361 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      421 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      481 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      541 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      601 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      661 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaaggatc
      721 cactagttct agagtcgaca tgcagcagaa cagtgagttc ttaacggaaa cacctggaag
      781 cgaccctcat atatctcaat tgcacgcgaa tagcgtaatg gaatcacagc tcttggacga
      841 tttcctcctg aacgggtctc ccatgtacca ggatgatagc atggcgcata ttaatattga
      901 tgagggtgct aatttccaaa atttt
//
