LOCUS       sequence-91957-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Hitchcock AL, Krebber H, Frietze S, Lin A, Latterich M, Silver PA
  TITLE     The conserved npl4 protein complex mediates proteasome-dependent 
            membrane-bound transcription factor activation.
  JOURNAL   Mol Biol Cell 2001 Oct;12(10):3226-41.
  PUBMED    11598205
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2001-10"; volume: "12(10)"; pages: "3226-41"
COMMENT     Sequence Label: pPS2351
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        143..807
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    359..476
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     771..794
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(847..875)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aggttatcag caacaacaca gtcatatcca ttctcaatta gctctaccac agtgtgtgaa
       61 ccaatgtatc cagcaccacc tgtaaccaaa acaattttag aagtactttc actttgtaac
      121 tgagctgtca tttatattga attttcaaaa attcttactt tttttttgga tggacgcaaa
      181 gaagtttaat aatcatatta catggcatta ccaccatata catatccata tacatatcca
      241 tatctaatct tacttatatg ttgtggaaat gtaaagagcc ccattatctt agcctaaaaa
      301 aaccttctct ttggaacttt cagtaatacg cttaactgct cattgctata ttgaagtacg
      361 gattagaagc cgccgagcgg gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct
      421 cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca
      481 ataaagattc tacaatacta gcttttatgg ttatgaagag gaaaaattgg cagtaacctg
      541 gccccacaaa ccttcaaatg aacgaatcaa attaacaacc ataggatgat aatgcgatta
      601 gttttttagc cttatttctg gggtaattaa tcagcgaagc gatgattttt gatctattaa
      661 cagatatata aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt
      721 ttgtattact tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc
      781 tatactttaa cgtcaaggag aaaaaacccc ggatctatga gtaaaggaga agaactcttc
      841 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      901 gtca
//
