LOCUS       sequence-91974-e        6543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Forced expression of human FOXH1GR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, 
            Nakamura M, Sutou K, Osafune K, Yamanaka S
  TITLE     Induction of pluripotency in human somatic cells via a transient 
            state resembling primitive streak-like mesendoderm.
  JOURNAL   Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
  PUBMED    24759836
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-24"; volume: "5:3678. doi"; pages: " 
            10.1038/ncomms4678"
COMMENT     Sequence Label: pMXs-hFOXH1GR
FEATURES             Location/Qualifiers
     source          1..6543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             4048..4517
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4647..4664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4818..5406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4898..4917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5570..6361,6362..6430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6193..6212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6431..6535)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggacctaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatcactg attttatccg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttac ctcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcgccctt caccatgggt ccctgcagcg gctcccgcct
     1981 ggggccccca gaggcagagt cgccctccca gccccctaag aggaggaaga agaggtacct
     2041 gcgacatgac aagcccccct acacctactt ggccatgatc gccttggtga ttcaggccgc
     2101 tccctcccgc agactgaagc tggcccagat catccgtcag gtccaggccg tgttcccctt
     2161 cttcagggaa gactacgagg gctggaaaga ctccattcgc cacaaccttt cctccaaccg
     2221 atgcttccgc aaggtgccca aggaccctgc aaagccccag gccaagggca acttctgggc
     2281 ggtcgacgtg agcctgatcc cagctgaggc gctccggctg cagaacaccg ccctgtgccg
     2341 gcgctggcag aacggaggtg cgcgtggagc cttcgccaag gacctgggcc cctacgtgct
     2401 gcacggccyg ccataccggc cgcccagtcc cccgccacca cccagtgagg gcttcagcat
     2461 caagtccctg ctaggagggt ccggggaggg ggcaccctgg ccggggctag ctccacagag
     2521 cagcccagtt cctgcaggca cagggaacag tggggaggag gcggtgccca ccccacccct
     2581 tccctcttct gagaggcctc tgtggcccct ctgccccctt cctggcccca cgagagtgga
     2641 gggggagact gtgcaggggg gagccatcgg gccctcaacc ctctccccag agcctagggc
     2701 ctggcctctc cacttactgc agggcaccgc agttcctggg ggacggtcca gcgggggaca
     2761 cagggcctcc ctctgggggc agctgcccac ctcctacttg cctatctaca ctcccaatgt
     2821 ggtaatgccc ttggcaccac cacccacctc ctgtccccag tgtccgtcaa ccagccctgc
     2881 ctactggggg gtggcccctg aaacccgagg gcccccaggg ctgctctgcg atctagacgc
     2941 cctcttccaa ggggtgccac ccaacaaaag catctacgac gtttgggtca gccaccctcg
     3001 ggacctggcg gcccctggcc caggctggct gctctcctgg tgcagcctga ctagtgccat
     3061 ggaaaatcct ggtaacaaaa caatagttcc tgcaacgtta ccacaactca cccctaccct
     3121 ggtgtcactg ttggaggtta ttgaacctga agtgttatat gcaggatatg atagctctgt
     3181 tccagactca acttggagga tcatgactac gctcaacatg ttaggagggc ggcaagtgat
     3241 tgcagcagtg aaatgggcaa aggcaatacc aggtttcagg aacttacacc tggatgacca
     3301 aatgacccta ctgcagtact cctggatgtt tcttatggca tttgctctgg ggtggagatc
     3361 atatagacaa tcaagtgcaa acctgctgtg ttttgctcct gatctgatta ttaatgagca
     3421 gagaatgact ctaccctgca tgtacgacca atgtaaacac atgctgtatg tttcctctga
     3481 gttacacagg cttcaggtat cttatgaaga gtatctctgt atgaaaacct tactgcttct
     3541 ctcttcagtt cctaaggacg gtctgaagag ccaagagcta tttgatgaaa ttagaatgac
     3601 ctacatcaaa gagctaggaa aagccattgt caagagggaa ggaaactcca gccagaactg
     3661 gcagcggttt tatcaactga caaaactctt ggattctatg catgaagtgg ttgaaaatct
     3721 ccttaactat tgcttccaaa catttttgga taagaccatg tccatcgagt tccccgagat
     3781 gttagctgaa atcatcacca atcagatacc aaaatattca aatggaaata tcaaaaaact
     3841 tctgtttcat caaaagtgag cggccgccag cacagtggtc gacgataaaa taaaagattt
     3901 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     3961 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca
     4021 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     4081 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
     4141 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     4201 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     4261 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     4321 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca
     4381 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
     4441 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     4501 tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt
     4561 atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgcgg
     5521 ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6241 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6301 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6361 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6421 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6541 ttc
//
