LOCUS       sequence-91977-e        7308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Forced expression of human GLIS1GR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, 
            Nakamura M, Sutou K, Osafune K, Yamanaka S
  TITLE     Induction of pluripotency in human somatic cells via a transient 
            state resembling primitive streak-like mesendoderm.
  JOURNAL   Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
  PUBMED    24759836
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-24"; volume: "5:3678. doi"; pages: " 
            10.1038/ncomms4678"
COMMENT     Sequence Label: pMXs-hGLIS1GR
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             4813..5282
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5412..5429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5583..6171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5663..5682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6335..7126,7127..7195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6958..6977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7196..7300)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggacctaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatcactg attttatccg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttac ctcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcgccctt caccatggca gaggcccgca catccctgtc
     1981 tgcccactgt cggggcccgc tggccactgg cctgcaccca gacctggacc tcccgggccg
     2041 aagcctcgcc acccctgcgc cttcctgcta ccttctgggc agcgaaccca gctctggcct
     2101 gggcctccag cccgagaccc acctccccga gggcagcctg aagcggtgct gtgtcttggg
     2161 cctacccccc acctccccag cctcctcctc accctgtgcc tcctccgacg tcacctccat
     2221 catccgctcc tcccagacgt ctctggtcac ctgtgtaaat ggactccgga gcccccctct
     2281 gacgggagat ctggggggcc cttccaagcg ggcccggcct ggccctgcat cgacggacag
     2341 ccatgagggc agcttgcaac ttgaagcctg ccggaaggcg agcttcctga agcaggaacc
     2401 cgcggatgag ttttcagagc tctttgggcc tcaccagcag ggcctgccgc ccccctatcc
     2461 cctgtctcag ttgccgcctg gcccaagcct tggaggcctg gggctgggcc tggcaggcag
     2521 ggtggtggcc gggcggcagg cgtgccgctg ggtggactgc tgtgcagcct atgagcagca
     2581 ggaggagctg gtgcggcaca tcgagaagag ccacatcgac cagcgcaagg gcgaggactt
     2641 cacctgcttc tgggctggct gcgtgcgccg ctacaagccc ttcaacgccc gctacaagct
     2701 gctcatccac atgcgagtgc actcgggcga gaagcccaac aagtgcatgt ttgaaggctg
     2761 cagcaaggcc ttctcacggc tggagaacct caagatccac ctgaggagcc acacgggcga
     2821 gaagccgtac ctgtgccagc acccgggttg ccagaaggcc ttcagcaact ccagcgaccg
     2881 cgccaagcac cagcgcaccc acctagacac gaagccgtac gcctgtcaga tccctggctg
     2941 ctccaagcgc tacacagacc ccagctccct ccgcaagcac gtcaaggccc attcagccaa
     3001 agagcagcag gtgcgtaaga agctgcatgc gggccctgac accgaggccg acgtcctgac
     3061 cgagtgtctg gtcctgcagc agctccacac gtccacacag ctggctgcca gcgacggcaa
     3121 gggtggctgt ggcctgggcc aggagctgct cccaggtgtg tatcctggct ccatcacccc
     3181 ccataacgga cttgcatcgg gcctcctgcc cccagcgcac gacgtacctt ccaggcacca
     3241 cccgctggat gccaccacca gttcccacca ccatctgtcc cctctgccca tggctgagag
     3301 cacccgggat gggttggggc ccggcctcct ctcaccaata gtcagccccc tgaaggggct
     3361 ggggccaccg ccgctgcccc catcctctca gagccattct ccggggggcc agcccttccc
     3421 cacactcccc agcaagccgt cctacccacc cttccagagc cctccacccc cgcctctgcc
     3481 cagcccacaa ggttaccagg gcagtttcca ctccatccag agttgcttcc cctatggcga
     3541 ctgctaccgg atggctgaac cagcagccgg tggggacgga ctggtcgggg agacccacgg
     3601 tttcaacccc ctgcggccca atggctacca cagcctcagc acgcccttgc ctgccacagg
     3661 ctatgaggcc ctggctgagg cctcatgccc cacagcgctg ccacagcagc catctgaaga
     3721 tgtggtgtcc agcggccccg aggactgtgg cttcttcccc aatggagcct ttgaccactg
     3781 cctgggccac atcccctcca tctacacaga caccactagt gccatggaaa atcctggtaa
     3841 caaaacaata gttcctgcaa cgttaccaca actcacccct accctggtgt cactgttgga
     3901 ggttattgaa cctgaagtgt tatatgcagg atatgatagc tctgttccag actcaacttg
     3961 gaggatcatg actacgctca acatgttagg agggcggcaa gtgattgcag cagtgaaatg
     4021 ggcaaaggca ataccaggtt tcaggaactt acacctggat gaccaaatga ccctactgca
     4081 gtactcctgg atgtttctta tggcatttgc tctggggtgg agatcatata gacaatcaag
     4141 tgcaaacctg ctgtgttttg ctcctgatct gattattaat gagcagagaa tgactctacc
     4201 ctgcatgtac gaccaatgta aacacatgct gtatgtttcc tctgagttac acaggcttca
     4261 ggtatcttat gaagagtatc tctgtatgaa aaccttactg cttctctctt cagttcctaa
     4321 ggacggtctg aagagccaag agctatttga tgaaattaga atgacctaca tcaaagagct
     4381 aggaaaagcc attgtcaaga gggaaggaaa ctccagccag aactggcagc ggttttatca
     4441 actgacaaaa ctcttggatt ctatgcatga agtggttgaa aatctcctta actattgctt
     4501 ccaaacattt ttggataaga ccatgtccat cgagttcccc gagatgttag ctgaaatcat
     4561 caccaatcag ataccaaaat attcaaatgg aaatatcaaa aaacttctgt ttcatcaaaa
     4621 gtgagcggcc gccagcacag tggtcgacga taaaataaaa gattttattt agtctccaga
     4681 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     4741 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     4801 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4861 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     4921 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     4981 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     5041 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     5101 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     5161 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     5221 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     5281 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     5341 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6001 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa
     6301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttc
//
