LOCUS       sequence-91984-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector has been discontinued as of 5/8/2014. See Comments below
            for information on the updated version of the lentiCRISPR vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, 
            Heckl D, Ebert BL, Root DE, Doench JG, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout screening in human cells.
  JOURNAL   Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. 
            Epub 2013 Dec 12.
  PUBMED    24336571
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1247005"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "84-7"
COMMENT     Sequence Label: lentiCRISPR
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..56
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             63..83
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acaggtgtcg tgacgcggga tccgccacca tggattacaa agacgatgac gataagatgg
       61 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
      121 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      181 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      241 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      301 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      361 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
      421 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
      481 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
      541 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
      601 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
      661 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
      721 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
      781 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
      841 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
      901 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
      961 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg a
//
