LOCUS       sequence-91988-e         504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent indicator for calcium imaging in the mitochondria 
            (low affinity variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV CEPIA4mt
FEATURES             Location/Qualifiers
     source          1..504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    102..326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     315..332
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(368..397)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtgggg
       61 atacccccta gagccccagc tggttctttc cgcctcagaa gccatagagc ccgggccatc
      121 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      181 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattanga
      241 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      301 agatggctgg caactagaag gcacagtcga ggctgatcag cgagcttcta gactatgcgg
      361 ccccattcag atcctcttct gagatgagtt tttgttctgc ggccgccttc gctgtcatca
      421 tttgtacaaa ctcttcgtag tttacctgac cttctccatc gatatctgct tccctgatca
      481 tttcatcaac ctcttcatct gtta
//
