LOCUS       sequence-91999-e        9964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for baculovirus expression of CAMSAP2 in Sf9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pFasBac+GFP-CAMSAP2
FEATURES             Location/Qualifiers
     source          1..9964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..28
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     262..279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  411..635
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(702..1235)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1424..1429,1430..1446,1447..1452))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1804..1895
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1820..1837
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1962..1979
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2001..2021
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2025..2027,2028..2738)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2025..2738
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2055..2083)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2678..2698
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     RBS             5785..5793
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7354..7488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7391..7410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7445..7464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  7517..7682
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7866..8321
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7953..7972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8163..8184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8348..8452
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8453..8521,8522..9313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8671..8690)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9484..9964,1..108)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
       61 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      301 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
      361 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
      421 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
      481 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
      541 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
      601 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
      661 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
      721 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
      781 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
      841 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
      901 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
      961 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     1021 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     1081 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     1141 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     1201 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     1261 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     1321 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     1381 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     1441 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     1501 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     1561 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     1621 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     1681 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     1741 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     1801 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     1861 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     1921 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
     1981 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatgagc aaaggagaag
     2041 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     2101 aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat
     2161 ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct
     2221 atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga
     2281 gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct
     2341 acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa
     2401 agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag tacaacttta
     2461 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
     2521 aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata
     2581 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg
     2641 tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
     2701 ctgctgggat tacacatggc atggatgagc tctacaaagg atccggggat gctgcagacc
     2761 ccagggagat gagaaagacg ttcattgttc cagccatcaa gccttttgac cactatgatt
     2821 tctccagggc caaaatcgcc tgcaatctgg cctggctggt ggccaaagcc tttgggacag
     2881 aaaatgtgcc agaggaactt caagaaccat tttacacaga tcagtatgac caggaacaca
     2941 tcaaaccacc tgttgttaat ttgcttctat cggctgaact atactgtcgt gctgggagtc
     3001 tcattctcaa gagtgatgct gcaaaacccc ttttgggcca tgatgctgta atccaggctt
     3061 tagcacagaa aggtctttat gtcactgacc aggaaaaatt ggtaactgaa cgagatctcc
     3121 acaagaaacc catacagatg agtgcacatt tggccatgat cgataccctc atgatggctt
     3181 atactgtaga aatggtcagt atagaaaaag taattgcgtg tgctcagcag tattcagctt
     3241 tttttcaagc cacagatctg ccctatgata ttgaggacgc tgtcatgtac tggataaata
     3301 aggtaaatga acatttgaaa gacataatgg aacaagaaca aaaactgaaa gaacatcaca
     3361 cagttgaagc tccaggaggt caaaagtctc cttccaaatg gttttggaaa ctggttccag
     3421 ctcgttatcg gaaagagcaa acattgctta agcaactgcc ttgcattcca ttggtagaaa
     3481 atttgttgaa ggatgggaca gatggctgtg cattagctgc ccttattcat ttttactgtc
     3541 ctgatgttgt cagattagag gatatttgtt tgaaagaaac tatgtctttg gctgatagcc
     3601 tgtataatct gcagctgatt caagaatttt gccaagaata cttgaaccag tgttgccatt
     3661 tcactctgga agatatgctc tatgctgctt catccataaa gagtaattat ttggtgttca
     3721 tggcggaact gttctggtgg tttgaagtgg tgaagccgtc ttttgtacag cctcgtgttg
     3781 ttcgtccaca aggagctgaa cctgtaaaag atatgccttc aattcctgtc ttgaatgctg
     3841 ccaaaagaaa tgtcttagat agtagttctg acttcccttc aagtggggaa ggagctacat
     3901 ttacacagtc tcatcatcat ttgccttcta ggtattcacg tccccaggct cattcttcag
     3961 cctcaggagg aattagaagg tcttcatcta tgtcttatgt tgatggcttc atagggacat
     4021 ggcccaaaga gaaaagatca tcagtgcatg gcgtatcatt tgatatttct tttgataaag
     4081 aagatagtgt acagagatcc actccaaacc gaggaatcac tcgttctatt agtaatgaag
     4141 gacttactct gaacaacagt catgtatcta aacacattag gaaaaatttg tccttcaagc
     4201 caataaatgg agaagaggaa gcagagagca ttgaagaaga acttaatata gattctcaca
     4261 gtgacctcaa atcttgtgtg ccccttaaca caaatgaact aaattctaat gagaatattc
     4321 attacaagct tccaaatgga gctttacaaa atagaatact tcttgacgag tttggcaatc
     4381 agatcgagac accaagcatt gaagaagcat tacaaataat tcatgatact gaaaaatctc
     4441 ctcatacacc tcagccagac caaattgcta atggcttctt tcttcatagt caagaaatga
     4501 gtatcttaaa ttcaaatatc aagttaaatc aatctagtcc tgataatgta actgatacga
     4561 aaggtgcctt gagtcccata actgacaata ctgaagtaga cactggaatt cacgttcctt
     4621 cagaagatat tcctgaaact atggacgaag attcttcgtt gagagattat actgtaagct
     4681 tggactctga catggatgat gcatctaaat ttcttcagga ttatgatatt cgaactggca
     4741 acaccaggga agctttgagt ccttgtccaa gtactgtaag taccaagtct cagccaggca
     4801 gcagtgcttc ttctagttct ggagttaaaa tgaccagctt tgctgaacaa aaattcagga
     4861 aactgaatca taccgatgga aaaagtagtg gaagcagttc tcaaaaaact acaccagaag
     4921 gctctgaact taatattcct catgtggttg cttgggcaca aattccagaa gaaacagggc
     4981 ttccacaggg acgggacact acccagctgt tggcctctga aatggtgcat cttaggatga
     5041 aactagaaga aaagaggcgt gctatagaag cccagaaaaa gaaaatggaa gctgctttta
     5101 ccaaacagag acagaaaatg ggaaggacag cattccttac tgtagtgaaa aagaaagggg
     5161 atgggatatc tcctctacga gaggaagcgg cgggtgcaga agatgagaaa gtatatactg
     5221 atcgagcaaa agaaaaggaa tcacaaaaaa ctgatggaca aaggagcaag tcactggcag
     5281 atataaaaga gagcatggag aatcctcaag ccaaatggct aaagtctcca actacaccta
     5341 ttgatcctga gaagcagtgg aacctggcaa gcccctcaga agaaacttta aatgaaggag
     5401 agattttaga atataccaaa tccattgaaa agttaaattc atccctgcat tttctacaac
     5461 aagaaatgca acgcttgtca cttcagcagg agatgttaat gcagatgaga gagcaacaat
     5521 cttgggtgat ttcacctcca caaccctctc cacagaaaca gattcgagat tttaagcctt
     5581 ctaagcaggc aggcctgtca tcagccattg caccattctc ctcagactcc cctcgtccta
     5641 ctcacccatc tccacagtct tctaacagga aaagtgcatc tttttctgtt aaaagtcaaa
     5701 ggactcctag gccaaatgag ttaaaaataa cacctttgaa tcgaaccttg acacctcctc
     5761 ggtctgtgga tagccttcct cggttaagga ggttttcacc aagtcaagtt cctattcaaa
     5821 ctaggtcatt tgtatgtttt ggggatgatg gagaacctca gttaaaggaa tccaaaccta
     5881 aagaggaagt taaaaaggag gaattggaat ccaaagggac tttggaacag cgtggacata
     5941 atccagaaga aaaggaaatc aaaccttttg agtcaacagt ctctgaagtc ctatcactgc
     6001 ctgtcacaga gactgtatgt ctgacaccaa atgaggacca attgaatcaa cccacagaac
     6061 cccctcctaa acccgttttc ccacccactg ctccaaaaaa tgttaatctg attgaagttt
     6121 ccctctcaga tttgaaaccc cctgaaaagg ctgatgtacc tgttgaaaaa tatgatggag
     6181 aaagtgataa agaacaattt gatgatgacc agaaagtatg ctgtggattc ttttttaagg
     6241 atgatcaaaa agcagaaaat gatatggcaa tgaaacgggc agctttgttg gagaaaagat
     6301 taagaaggga aaaggaaact cagctccgga aacaacagtt ggaagcagaa atggagcata
     6361 agaaggagga aacaaggcgt aaaactgagg aagaacgtca gaagaaagaa gatgagagag
     6421 cacgcagaga atttattagg caagaatata tgaggcggaa acaactgaaa ctaatggaag
     6481 atatggatac agtaattaaa ccccgtcctc aagtagtaaa acaaaaaaaa cagcgaccaa
     6541 aatctattca cagagatcat attgaatccc ccaaaacacc aataaagggt cctccagtct
     6601 ctagcctttc tttggcatcg ctgaacacgg gtgataacga gagtgtacat tcaggcaaga
     6661 ggacgccaag atcagagtct gtagaaggct tcttatctcc aagtcgttgt ggcagtcgaa
     6721 atggagaaaa agactgggag aatgcatcaa caacttcttc agtggcttct ggaacagaat
     6781 atacaggacc aaagctctac aaagaaccca gtgcaaaatc caataagcac ataatacaaa
     6841 atgctttagc tcattgctgt ttggctggaa aagtaaatga aggtcagaag aaaaaaatac
     6901 tggaggaaat ggagaaatca gatgccaaca acttcttaat cttgttccgg gattcaggat
     6961 gccagttcag atctttatac acttattgcc cagaaactga agaaatcaat aaactgactg
     7021 ggataggccc taaatctatc actaaaaaaa tgattgaagg actttacaaa tataattctg
     7081 acaggaaaca gtttagccac atacccgcta aaactttatc tgccagtgtt gatgcaatta
     7141 ccattcatag ccatttatgg cagaccaaaa gaccagtaac acccaaaaaa cttttaccca
     7201 ctaaggcata gggcatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc
     7261 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     7321 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     7381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     7501 gagcctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     7561 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     7621 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     7681 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     7741 caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc
     7801 ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga
     7861 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7921 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7981 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8041 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8101 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8161 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8221 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8281 atttaacgcg aattttaaca aaatattaac gtttacaatt tcaggtggca cttttcgggg
     8341 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8401 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8461 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8521 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8581 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8641 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8701 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8761 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8821 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8881 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8941 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9001 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9061 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9121 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9181 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9241 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9301 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9361 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9421 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9601 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     9661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9841 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     9901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9961 ggag
//
