LOCUS       sequence-92-expo        7227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_10
FEATURES             Location/Qualifiers
     source          1..7227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(254..276)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4667..4689)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4789..4808
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4886..4904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4972..5076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5077..5145,5146..5937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5295..5314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6108..6696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6597..6616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6850..6867
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6984..7005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7020..7025,7026..7043,7044..7050)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7058..7074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7063..7085
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7082..7098
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7082..7098
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagagg atccccggga ttggccaaag gacccaaagg
       61 tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct tggagggtac
      121 cgagctcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggtg ggtgaagacc
      181 agaaacagca cctcgaactg agccgcgata ttgcccagcg tttcaacgcg ctgtatggcg
      241 agatcgatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      301 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      361 atcgcccttc ccaacagttg cgaaggtaag tttaaacaga tccatactaa ctaacttgtt
      421 ctgacataat tttcagcttg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
      481 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
      541 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
      601 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
      661 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
      721 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggta agtttaatta
      781 agttgatact aactaacaag atctgattaa ttttcaggac agtcgtttgc cgtctgaatt
      841 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
      901 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
      961 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
     1021 taatgatgat ttctcccgcg ctgtactgga ggctgaagtc caggtaagtt taaacaggat
     1081 cttactaact aacatgctaa cactgaattt tcagatgtgc ggcgagttgc gtgactacct
     1141 acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt
     1201 cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa
     1261 cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga
     1321 actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga
     1381 ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt
     1441 taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca
     1501 ggatgtaagt ttaaactatt cgttactaac taactttaaa catttaaatt ttcagatcct
     1561 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     1621 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     1681 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     1741 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     1801 gctggggaat ggtaagttta aacagttgaa tactaactaa cggagatctt tgaaattttc
     1861 agaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     1921 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     1981 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     2041 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg attctttgcg aggtaagttt
     2101 aaacagaact ctactaacta acacattaga tcctaatttt cagtacgctc acgcgatggg
     2161 caacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca
     2221 gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa
     2281 cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat
     2341 gaacggtctg gtctttgccg accgcacgcc gcatccagcg taagtttaaa caataaccta
     2401 ctaactaacg tagataattt aaattttcag gctgacggaa gcaaaacacc agcagcagtt
     2461 tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca
     2521 tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg
     2581 tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc
     2641 gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac
     2701 cgcatggtca gaagccgggc acatcagcgc atggcagcag tggaggtaag tttaaacaag
     2761 atcctactaa ctaactctac attgatgaat tttcagactg gcggaaaacc tcagtgtgac
     2821 gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat
     2881 cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg
     2941 gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct
     3001 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcggtaa
     3061 gtttaaacaa agttgtacta actaacgaag atcttgataa ttttcagaac gctggaaggc
     3121 ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
     3181 tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
     3241 ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
     3301 ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
     3361 agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
     3421 cgcctgtttt gaccgctggg atctgccatt gtcagacatg tagtaagttt aaacttgata
     3481 gtactaacta acatgtttca tttaaatttt cagtaccccg tacgtcttcc cgagcgaaaa
     3541 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
     3601 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct
     3661 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
     3721 cgacgactcc tggagcccgt cagtatcggc ggaattccaa ctgagcgccg gtcgctacca
     3781 ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac
     3841 tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg
     3901 acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     3961 ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta
     4021 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     4081 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     4141 tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga
     4201 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     4261 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     4321 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     4381 gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa
     4441 aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc
     4501 tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt
     4561 gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct actagtcggc
     4621 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4681 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4741 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     4801 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4861 aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc
     4921 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5041 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5101 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5161 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5221 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5281 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5341 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5401 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5461 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5521 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5581 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5641 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5701 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5761 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5821 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5881 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6241 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6481 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6841 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6901 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6961 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7021 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7081 acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg atcttactaa
     7141 ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg
     7201 gatacgctaa caacttggaa atgaaat
//
