LOCUS       sequence-92000-e        9267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for baculovirus expression of mouse CAMSAP3 in Sf9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pFasBac+GFP-CAMSAP3
FEATURES             Location/Qualifiers
     source          1..9267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..28
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     262..279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  411..635
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(702..1235)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1424..1429,1430..1446,1447..1452))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1804..1895
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1820..1837
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1962..1979
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2001..2021
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2034..2036,2037..2747)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2034..2036,2037..2747)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2076..2097)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2337..2356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2684..2705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6657..6791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6694..6713)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6748..6767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6820..6985
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7169..7624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7256..7275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7466..7487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7651..7755
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7756..7824,7825..8616)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7974..7993)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8787..9267,1..108)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
       61 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      301 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
      361 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
      421 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
      481 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
      541 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
      601 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
      661 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
      721 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
      781 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
      841 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
      901 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
      961 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     1021 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     1081 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     1141 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     1201 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     1261 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     1321 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     1381 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     1441 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     1501 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     1561 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     1621 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     1681 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     1741 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     1801 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     1861 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     1921 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
     1981 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggat ccagtgagca
     2041 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2101 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2161 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2221 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2281 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2341 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2401 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2461 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2521 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2581 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2641 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2701 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggag ctcgtggaag
     2761 cggcgcccgc agggtccggg ccactgcgga ggactttcct ggtccccgag atcaagtctt
     2821 tggaccagta cgatttctct cgagccaagg cggcggcgag tctggcgtgg gtgctgcggg
     2881 ccgcattcgg gggagcagag catgtgcctc cggagctgtg ggaacccttc tacaccgatc
     2941 agtacgcaca ggagcacgtg aagcccccag tgacgcggct gctgctctct gcggaactat
     3001 actgtcgggc ctggcgccag gcactgccac agctcgagcc atcccccagt ccctctgctc
     3061 tgttggcctt gctggcgagg aggggcacgg tgccttcact gcctgagcac ccagtgcgcg
     3121 aggctgacct gaaacaccag ccaattctca tgggagccca cctagctgtc atcgatgctc
     3181 tcatggttgc cttctcattt gagtggacaa agacactacc tggtcccttg gctctgagca
     3241 gcttggagca caaactcctt ttctgggtag acacaactgt tcggcggctg caggagaaga
     3301 cagaacaaga agcagcccag cgtgcatctc ctgcagcccc tctggatggg gcctctcctg
     3361 cccagccctc gattcgatat cgcaaggacc gtgccattgc ccgaagggct ccctgctttc
     3421 caaatgtgac tacccttcag gacctggcca gtggggcagc actggctgcc accatccact
     3481 gctattgtcc ccagctatta cgacttgagg aggtgtgcct caaggacccc atgtctgtgg
     3541 cagacagtct atacaacctc cagctggtgc aagacttctg tgcctcccat cttcctcgag
     3601 gctgccccct gtcccttgaa gacttgctgt atgtcccacc acccctcaag gtcaacctgg
     3661 tggtgctgtt ggctgagatg tatatgtgct ttgaggttct gaagcctgat tttgtgcagg
     3721 ccaaagactt gcctgatgga catgctgtct ccccccggaa tacagagact gttccatctc
     3781 agaacaacag tggcagcagt tctcctgtct tcaacttccg tcacccactt ctgtcacctg
     3841 gtggtcccca gtctccactc cgaggatcca caggctccct gaagtcctct ccatcaatgt
     3901 ctcacatgga ggctcttggc aaagcctgga accgtcagct tagccgtccc ctctcccagg
     3961 ctgtgtcgtt cagcactccc tttggcctgg acagcgatgt ggatgtcgtc atgggagatc
     4021 ctgtcctgct ccgctccgtc agctcagaca gtctgggtcc tccacgtcct gtgtcaacat
     4081 catcccggaa ttctgctcag ccagccccag aatctggaga cctacccacg attgaagagg
     4141 ccctgcagat cattcacagt gctgagcccc gactgctccc tgatggggct gctgatggca
     4201 gtttctacct ccattctcct gagggtctct ccaaaccacc actctccccc taccctcccg
     4261 aaggagcctc aaagccgctg tctgataggc tcaacaaggc gcctatctat atatcacacc
     4321 ctgagaaccc ttcaaaatca tctccctgct caacaggaga gatactgaaa ccaccacccc
     4381 catccgaggg ttcccccaaa gctgtggctt catccccagc agccaacaac tccgaagtga
     4441 agatgaccag ttttgctgaa cgcaagaaac agctggtgaa ggctgaggct gagtcaggat
     4501 tggggtctcc aacatccacc cccgtagcac ctgaggcctt gagctcagag atgagtgagc
     4561 tgggagccag gctggaggag aagcgccgag ccatagaggc acagaaacga cgcattgagg
     4621 caatctttgc caagcatagg cagaggctgg gcaagagcgc tttcctgcag gtgcagcctc
     4681 gggaggctgc aggggaggcc gaggaggaag ctgagctggg ctcagttcct ggtggggaac
     4741 ggccagcagg tgagggccag ggtgagccgt ccttacggca caagtcagtt accttctctc
     4801 cagacctggg cccagtgccc ccagagggac ttggggatta caatagagca gtcagtaagc
     4861 tgagtgccgc tctgagctct ctgcagcggg acatgcagag gctcacagac cagcaacagc
     4921 ggcttctagc ccctccagaa gctcctggac ctgccccacc acctgcagcc tgggtcattc
     4981 ctggacccgc cactgggcct aaagcagcat cccccagccc tgcccgtcgt gccccagctg
     5041 cccgacgcag ccctgggcca ggccccagcc caacaccccg tagtccaaaa catgcaaggc
     5101 cggcagagct gaagcttgca cctttgacaa gggtactcac accaccccat gatgtagaca
     5161 gcctccctca cctacgcaag ttttcaccca gccaggtgcc tgtacagact cgctcctcta
     5221 tcctcctgtc ggaggggaca cctcccgagg agcccaccac caagcctgcc ctcattgaga
     5281 tccctctagc cagcctgggg gagcctgctg ctgatgagga aggagatggg agcccccctg
     5341 gggctgagga ttccttagag gaagaggcat cttctgaggg agagccccga tcagggcttg
     5401 gattctttta taaggacgaa gacaagcctg aggatgagat ggctcagaag cgagctagcc
     5461 tgctggagcg tcagcagagg cgggtagagg aagcccggcg gcgcaaacag tggcaggagg
     5521 cagagaagga gcagaaacgg gaggaggccg ccaggctggc tcaggaggct ccaggcttgg
     5581 cctttacaac tcctgttgta gcctctgcgg ctccagtggc caccttggct cctactacca
     5641 gagccatggc cccagctgag gaggaggtgg gcccccggcg gggggacttc acaagacttg
     5701 agtatgaacg ccgggcacaa ctgaaactga tggatgacct tgataaggtg ctacggcccc
     5761 gggcctcagg gaccggggga ccagggcggg gcgggcgcag ggccacccgg ccacgctctg
     5821 gttgctgtga tgactcggcc ttggcacgaa gcccagcccg cggcctgctg ggttcacggc
     5881 tcagcaaggt ctattcccag tccacgctgt ccctatctac ggtggccaac gaggctccca
     5941 ataaccttgg tgtgaagagg cccacctctc gggccccttc tccatcaggc ctcatgtctc
     6001 caagccgcct gcctggcagt cgagaacgtg actgggagaa tggaagcaat gcatcctccc
     6061 cagcatcagt gcctgagtac acaggtcccc ggctatacaa ggaacccagc gccaagtcta
     6121 acaagtttat catccacaat gccttgtcac actgctgcct ggcaggcaag gtgaatgagc
     6181 cccagaagaa cagaattcta gaggaaatcg agaaaagcaa ggccaaccac ttcctgattc
     6241 tctttcggga ctcaagctgc cagttccggg ccctctacac tctgtctggg gagacagagg
     6301 agctatcgag gctggcaggc tatgggcctc ggaccgtcac tcctgccatg gtcgaaggca
     6361 tctataagta caactcggat cgcaaacggt tcacccagat ccctgctaaa accatgtcta
     6421 tgagtgtaga cgccttcact atccagggac acctttggca aagcaagaag cccaccacgc
     6481 ccaagaaggg tggcggtacc cccaaatagc tcgaggcatg cggtaccaag cttgtcgaga
     6541 agtactagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     6601 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     6661 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6721 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6781 atgtctggat ctgatcactg cttgagccta ggagatccga accagataag tgaaatctag
     6841 ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc
     6901 catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag
     6961 ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttttaatca
     7021 aacatcctgc caactccatg tgacaaaccg tcatcttcgg ctactttttc tctgtcacag
     7081 aatgaaaatt tttctgtcat ctcttcgtta ttaatgtttg taattgactg aatatcaacg
     7141 cttatttgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7201 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7261 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7321 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7381 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7441 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7501 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7561 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     7621 atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7981 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8281 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8461 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     9181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9241 gtcggaacag gagagcgcac gagggag
//
