LOCUS       sequence-92011-e         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent indicator for calcium imaging in the mitochondria 
            (moderately low affinity variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV CEPIA3mt
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..119
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             141..215
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             255..272
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             276..308
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(312..320,321..335)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      342..351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             363..419
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 ctttctggct aactagagaa cccactgctt actggcacgt gaccatgtcc gtcctgacgc
       61 cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaaa
      121 ttcattcact gggggacccc atgagcgtgc tcaccccact cctgctgcgg gggctgaccg
      181 gcagcgctag gcggctgcca gtcccgcggg ccaagatcca cagtctcggc gatcccggat
      241 ccatgcgggg ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc
      301 aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat
      361 cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac
      421 tcgagaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga
      481 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc
      541 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaaac
      601 tttcg
//
