LOCUS       sequence-92013-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent indicator for calcium imaging in the endoplasmic 
            reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV G-CEPIA1er
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     join(62..106,189..200)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             join(62..107,190..200)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             237..293
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     506..527
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctggctaac tagagaaccc actgcttact ggcacgtgga aattaatacg acgtggccac
       61 catgggatgg agctgtatca tcctcttctt ggtagcaaca gctacaggta aggggttaac
      121 agtagcaggc ttgaggtctg gacatatata tgggtgacaa tgacatccac tttgcctttc
      181 tctccacagg cgcgcactcc caggtccaac tgcagggatc catggtcgac tcttcacgtc
      241 gtaagtggaa taagacaggt cacgcagtca gagctatagg tcggctgagc tcactcgaga
      301 acgtctatat caaggccgac gagcagaaga acggcatcaa ggcgtacttc aagatccgcc
      361 acaacatcga gggcggcggc gtgcagctcg cctaccacta ccagcagaac acccccatcg
      421 gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtgcagtcc atactttcga
      481 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      541 tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg
      601 gcgaggagct gttcaccggg gtggtgccca tccaggtcga gctggacggc gacgtaaacg
      661 gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc aagctgaccc
      721 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      781 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      841 tcaagtccgc catgcccgaa ggctacatcc aggagcgcac
//
