LOCUS       sequence-92014-e        3396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of Patronin CC truncation (using 
            Baculodirect kit from Invitrogen).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pENTR-GFP-Patronin CC 638–1457
FEATURES             Location/Qualifiers
     source          1..3396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..777
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             67..777
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(106..127)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(367..386)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     714..735
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    787..811
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3209..3308)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3325..3344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3326..3344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3349..3365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3349..3365)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg cccccttcac
       61 catgggagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      121 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      181 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      241 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      301 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      361 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      421 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      481 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      541 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      601 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      661 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      721 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagcac
      781 cggtatacaa gtttgtacaa aaaagcaggc tccgcggccg cccccatgaa gtgggagacc
      841 atgagcaacg aatcaaagag aacacctgat atggatcccg tggatttgga caagtaccag
      901 caaagtatcg ccattatgaa catgaatctg caggatatcc agcaggatat ccaccgcctg
      961 gccacccagc aaagccaaat gcaggcgcag cacctccaag cccaacaact catgcaggct
     1021 cagcaaatag ccaacatgct gaaccagcag acctatgggt cgcagcagca cctggctgat
     1081 caccattacc agcagcagag acccatgcag caaagctttg gttcatcgcc ccatcttccg
     1141 caggcctaca acgccccagt cagcgcatac agctcccgtc cgcccagtcg cgatccctac
     1201 cagcagcagc tccaccatca gcagcagcag cccatgccca tgccgcaacc gatgcagtac
     1261 gtcaacgagc atgggcagta tatgtcgccg ccgcagcccg cacactacat gccgcagcag
     1321 gcacaacagc cgcagagcat ttacagcgac aacggggcgg cgtacaatca cagtaaccac
     1381 tcgccatacg gcggagctcc acagtatcgg agcagcgtgg tgtacgacga ttacgggcag
     1441 cccaccaacc acttctacct gcatgagtca tcgccgcagc ctcaagctca tcagcatccg
     1501 cagcgcagga cttgggctca ctccgcagca gccgccgctt atgaacaaca gcaacagatc
     1561 cagccttccc tggtggatgt gaatgcctgg cagacgcagc agcaccaaaa gcagaagcag
     1621 acctggatga acaggccgcc ctcgagtgcg ggagccccga gtcctggcag ctttatgctg
     1681 caccagaacg gtggaggagg aggtggtggc ggtggtggtg gcgagctaca gcacctgttt
     1741 caggtacagg cctcgccaca gcatggccaa cggcaggtta gtggatccaa tggcgtgcag
     1801 cgccagcagt cgttgaccaa cttgcgagac aatcgctcgc ccaaggcacc acaaaacatg
     1861 ggaatgccca tgggtatgcc aatgcagcaa gaggacatga tggcaccgca gagtatttgc
     1921 tttatcggtg acgaggagga tgttgatgag ctggagcgga acattatcga atctatgcag
     1981 tcgacgcaca tctccgactt tgtacaccaa cagcagcagc agcaccaaca ccaacagcaa
     2041 cttcagcagc aacaacggtt gcaggggcac agcggacgag gtagcagctc ggaggattat
     2101 gacagcgggg agatgatctc caacaagctg aacatcacca gcggcaatct cacctatcgc
     2161 ataccctcgc catcccgtcc ctccatccaa gccaacagct tccaggatcc ccgagccatg
     2221 gcagctgctt ccggtggcga ggaccagccg cccgagaagg gtttctacat ctccttcgac
     2281 gatgagcagc ccaaacgacc caagccacct ctgcgcgcca agcgatcgcc taaaaaggag
     2341 tctccaccag gaagtcggga cagcgtcgat aaccaggcga ccctgaaacg tgaatcgcta
     2401 agtcatctgc acaacaacaa caatattgga ttcggaaatg atgatgtcaa cagcaaaccg
     2461 gtgaccaggc acagtatcca tggcctaaac aactccaaca gtgtcaaatc tcccggaaat
     2521 gccacgtaca acaagtacac ggatgagccg cccatccaac tgcgacagtt ggccgtttcg
     2581 ggagcaatgt caccaactag taacgaacgt catcacttgg acgatgtcag caatcagtca
     2641 ccgcagcaga cgcagcaacc aatgtcgccc acgcgactcc aacagagcag caacaacgca
     2701 gaggcggcca agaacaaggc gctggtcatc ggagcagatt ccaccaattt ggatccggaa
     2761 tctgttgatg agatggagcg gcgcaaggag aaaatcatgc tgctgtcttt gcaacgtcgc
     2821 cagcaacagg aggaggcgaa ggcgcgcaaa gagattgagg cttcccagaa gcgagaaaag
     2881 gagcgcgaaa aggaggagga acgagcgcgg aaaaaggagg agcaaatggc acggcgagcg
     2941 gccattttgg agcagcacag actcaagaaa gccattgaag aggccgagcg agagggtaaa
     3001 accctggatc ggcccgatct gcacgtgaag ctgcaatcgc attcatccac ctcaacgacc
     3061 ccacgactga ggcagcagcg caccacgcgt cccaggccta agacaattca cgtggacgat
     3121 gccagcgtgg acatcagcga ggcttcaagc atctctagtc gaggcaaaaa aggctcaagc
     3181 tcgaatctaa ccaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata
     3241 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     3301 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     3361 tcctggcagc tctggcccgt gtctcaaaat ctctga
//
