LOCUS       sequence-92015-e        4521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of Patronin CC+CKK (using Baculodirect 
            kit from Invitrogen).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pENTR-GFP-Patronin CC+CKK
FEATURES             Location/Qualifiers
     source          1..4521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..28
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(79..81,82..84,85..795)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(79..81,82..84,85..795)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(124..145)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(385..404)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     732..753
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    805..829
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4334..4433)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4450..4469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4451..4469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4474..4490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4474..4490)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgccaactt tgtacaaaaa agcaggctcc gcggccgccg tactcgagga aaacctgtat
       61 tttcagggct ccttcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      121 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      181 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      241 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      301 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      361 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      421 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      481 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      541 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      601 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      661 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      721 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      781 gacgagctgt acaagcaccg gtatacaagt ttgtacaaaa aagcaggctc cgcggccgcc
      841 cccttcacca tggatngaaa tgtgcccacg gtgtcgtcgc acaatctttc gaatgcgggc
      901 agcccgttgc cggtggctag gcacaagcaa cattccagcg acaaagacta cagcagcaac
      961 agcggcatga caccagatgc atacaacgat tcccgctcca ccagtggcta tgatccggag
     1021 agcacgcccg ttcgcaagtc ctcgactagc agcatgccag caagtcccgc tgcctggcag
     1081 ttagatgtgg gagacgacga tatgcgctcg ctggaaaacg ccagcaagtt gtccacaatt
     1141 cgaatgaaac tggaggagaa gcggcggcgc attgagcagg acaagcgcaa gatcgagatg
     1201 gccttgctgc ggcaccagga gaaggaggat ttggaatcgt gtccggacgt gatgaagtgg
     1261 gagaccatga gcaacgaatc aaagagaaca cctgatatgg atcccgtgga tttggacaag
     1321 taccagcaaa gtatcgccat tatgaacatg aatctgcagg atatccagca ggatatccac
     1381 cgcctggcca cccagcaaag ccaaatgcag gcgcagcacc tccaagccca acaactcatg
     1441 caggctcagc aaatagccaa catgctgaac cagcagcaga cctatgggtc gcagcagcac
     1501 ctggctgatc accattacca gcagcagaga cccatgcagc aaagctttgg ttcatcgccc
     1561 catcttccgc aggcctacaa cgccccagtc agcgcataca gctcccgtcc gcccagtcgc
     1621 gatccctacc agcagcagct ccaccatcag cagcagcagc ccatgcccat gccgcaaccg
     1681 atgcagtacg tcaacgagca tgggcagtat atgtcgccgc cgcagcccgc acactacatg
     1741 ccgcagcagg cacaacagcc gcagagcatt tacagcgaca acggggcggc gtacaatcac
     1801 agtaaccact cgccatacgg cggagctcca cagtatcgga gcagcgtggt gtacgacgat
     1861 tacgggcagc ccaccaacca cttctacctg catgagtcat cgccgcagcc tcaagctcat
     1921 cagcatccgc agcgcaggac ttgggctcac tccgcagcag ccgccgctta tgaacaacag
     1981 caacagatcc agccttccct ggtggatgtg aatgcctggc agacgcagca gcaccaaaag
     2041 cagaagcaga cctggatgaa caggccgccc tcgagtgcgg gagccccgag tcctggcagc
     2101 tttatgctgc accagaacgg tggaggagga ggtggtggcg gtggtggtgg cgagctacag
     2161 cacctgtttc aggtacaggc ctcgccacag catggccaac ggcaggttag tggatccaat
     2221 ggcgtgcagc gccagcagtc gttgaccaac ttgcgagaca atcgctcgcc caaggcacca
     2281 caaaacatgg gaatgcccat gggtatgcca atgcagcaag aggacatgat ggcaccgcag
     2341 agtatttgct ttatcggtga cgaggaggat gttgatgagc tggagcggaa cattatcgaa
     2401 tctatgcagt cgacgcacat ctccgacttt gtacaccaac agcagcagca gcaccaacac
     2461 caacagcaac ttcagcagca acaacggttg caggggcaca gcggacgagg tagcagctcg
     2521 gaggattatg acagcgggga gatgatctcc aacaagctga acatcaccag cggcaatctc
     2581 acctatcgca taccctcgcc atcccgtccc tccatccaag ccaacagctt ccaggatccc
     2641 cgagccatgg cagctgcttc cggtggcgag gaccagccgc ccgagaaggg tttctacatc
     2701 tccttcgacg atgagcagcc caaacgaccc aagccacctc tgcgcgccaa gcgatcgcct
     2761 aaaaaggagt ctccaccagg aagtcgggac agcgtcgata accaggcgac cctgaaacgt
     2821 gaatcgctaa gtcatctgca caacaacaac aatattggat tcggaaatga tgatgtcaac
     2881 agcaaaccgg tgaccaggca cagtatccat ggcctaaaca actccaacag tgtcaaatct
     2941 cccggaaatg ccacgtacaa caagtacacg gatgagccgc ccatccaact gcgacagttg
     3001 gccgtttcgg gagcaatgtc accaactagt aacgaacgtc atcacttgga cgatgtcagc
     3061 aatcagtcac cgcagcagac gcagcaacca atgtcgccca cgcgactcca acagagcagc
     3121 aacaacgcag aggcggccaa gaacaaggcg ctggtcatcg gagcagattc caccaatttg
     3181 gatccggaat ctgttgatga gatggagcgg cgcaaggaga aaatcatgct gctgtctttg
     3241 caacgtcgcc agcaacagga ggaggcgaag gcgcgcaaag agattgaggc ttcccagaag
     3301 cgagaaaagg agcgcgaaaa ggaggaggaa cgagcgcgga aaaaggagga gcaaatggca
     3361 cggcgagcgg ccattttgga gcagcacaga ctcaagaaag ccattgaaga ggccgagcga
     3421 gagggtaaaa ccctggatcg gcccgatctg cacgtgaagc tgcaatcgca ttcatccacc
     3481 tcaacgaccc cacgactgag gcagcagcgc accacgcgtc ccaggcctaa gacaattcac
     3541 gtggacgatg ccagcgtgga catcagcgag gcttcaagca tctctagtcg gggcaaaaaa
     3601 ggctcaagct cgaatctaac cggctacggt caactaagct caaattcaat gaaaagagat
     3661 tactacaggg gctcgcaaga ctccctcact ataaaagatt cgggactggg acgcgccact
     3721 ccgccgaggc gtgctccgtc gcctggaatg ggaatgggcg cttcaggtag gcatatgcca
     3781 tctccctccg gaccgggctc attgccgcca ggtttgatat cgaaacgtcg cggatttgat
     3841 gatggatcca gcgatttctc tttaactccg aatttgaaca tggaatattc gggtcctaaa
     3901 ctctataaac aaccagcggc caaatcgaat cgtggaatca tcctgaatgc cgttgaatac
     3961 tgtgtttttc ccggcgttgt taaccgcgag gccaaacaga aagtgctgga gaagattgcg
     4021 cgctcggagg cgaagcactt cctggtactc ttccgcgatg ctggctgcca gttccgcgcc
     4081 ctctacagct accagcccga aacggaccag gtaaccaagc tgtatggtac tgggcctagt
     4141 caagtcgaag aagtcatgtt cgacaagttt ttcaaataca actcaggagg caagtgcttc
     4201 tcgcaagtgc atacaaagca tctgacagtg accatcgacg ccttcacaat acacaactcc
     4261 ctgtggcggg gcaagcgggt gcagttgccc agcaaaaaag acatggcgct tgtaatcaag
     4321 ggtgggcgcg cggagcctgc ttttttgtac aaagttggca ttataaaaaa gcattgctca
     4381 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     4441 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     4501 cccgtgtctc aaaatctctg a
//
