LOCUS       sequence-92016-e        3546 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of Patronin CC truncation (using 
            Baculodirect kit from Invitrogen).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pENTR-GFP-Patronin CC 534–1457
FEATURES             Location/Qualifiers
     source          1..3546
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    727..751
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagcac
      721 cggtatacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac catggttgac
      781 aatgtgccca cggtgtcgtc gcacaatctt tcgaatgcgg gcagcccgtt gccggtggct
      841 aggcacaagc aacattccag cgacaaagac tacagcagca acagcggcat gacaccagat
      901 gcatacaacg attcccgctc caccagtggc tatgatccgg agagcacgcc cgttcgcaag
      961 tcctcgacta gcagcatgcc agcaagtccc gctgcctggc agttagatgt gggagacgac
     1021 gatatgcgct cgctggaaaa cgccagcaag ttgtccacaa ttcgaatgaa actggaggag
     1081 aagcggcggc gcattgagca ggacaagcgc aagatcgaga tggccttgct gcggcaccag
     1141 gagaaggagg atttggaatc gtgtccggac gtgatgaagt gggagaccat gagcaacgaa
     1201 tcaaagagaa cacctgatat ggatcccgtg gatttggaca agtaccagca aagtatcgcc
     1261 attatgaaca tgaatctgca ggatatccag caggatatcc accgcctggc cacccagcaa
     1321 agccaaatgc aggcgcagca cctccaagcc caacaactca tgcaggctca gcaaatagcc
     1381 aacatgctga accagcagca gacctatggg tcgcagcagc acctggctga tcaccattac
     1441 cagcagcaga gacccatgca gcaaagcttt ggttcatcgc cccatcttcc gcaggcctac
     1501 aacgccccag tcagcgcata cagctcccgt ccgcccagtc gcgatcccta ccagcagcag
     1561 ctccaccatc agcagcagca gcccatgccc atgccgcaac cgatgcagta cgtcaacgag
     1621 catgggcagt atatgtcgcc gccgcagccc gcacactaca tgccgcagca ggcacaacag
     1681 ccgcagagca tttacagcga caacggggcg gcgtacaatc acagtaacca ctcgccatat
     1741 ggcggaactc cacagtatcg gagcagcgtg gtgtacgacg attacgggca gcccaccaac
     1801 cacttctacc tgcatgagtc atcgccgcag cctcaagctc atcagcatcc gcagcgcagg
     1861 acttgggctc actccgcagc agccgccgct tatgaacaac agcaacagat ccagccttcc
     1921 ctggtggatg tgaatgcctg gcagacgcag cagcaccaaa agcagaagca gacctggatg
     1981 aacaggccgc cctcgagtgc gggagccccg agtcctggca gctttatgct gcaccagaac
     2041 ggtggaggag gaggtggtgg cggtggtggt ggcgagctac agcacctgtt tcaggtacag
     2101 gcctcgccac agcatggcca acggcaggtt agtggatcca atggcgtgca gcgccagcag
     2161 tcgttgacca acttgcgaga caatcgctcg cccaaggcac cacaaaacat gggaatgccc
     2221 atgggtatgc caatgcagca agaggacatg atggcaccgc agagtatttg ctttatcggt
     2281 gacgaggagg atgttgatga gctggagcgg aacatcatcg aatctatgca gtcgacgcac
     2341 atctccgact ttgtacacca acagcagcag cagcaccaac accaacagca acttcagcag
     2401 caacaacggt tgcaggggca cagcggacga ggtagcagct cggaggatta tgacagcggg
     2461 gagatgatct ccaacaagct gaacatcacc agcggcaatc tcacctatcg cataccctcg
     2521 ccatcccgtc cctccatcca agccaacagc ttccaggatc cccgagccat ggcagctgct
     2581 tccggtggcg aggaccagcc gcccgagaag ggtttctaca tctccttcga cgatgagcag
     2641 cccaaacgac ccaagccacc tctgcgcgcc aagcgatcgc ctaaaaagga gtctccacca
     2701 ggaagtcggg acagcgtcga taaccaggcg accctgaaac gtgaatcgct aagtcatctg
     2761 cacaacaaca acaatattgg attcggaaat gatgatgtca acagcaaacc ggtgaccagg
     2821 cacagtatcc atggcctaaa caactccaac agtgtcaaat ctcccggaaa tgccacgtac
     2881 aacaagtaca cggatgagcc gcccatccaa ctgcgacagt tggccgtttc gggagcaatg
     2941 tcaccaacta gtaacgaacg tcatcacttg gacgatgtca gcaatcagtc accgcagcag
     3001 acgcagcaac caatgtcgcc cacgcgactc caacagagca gcaacaacgc agaggcggcc
     3061 aagaacaagg cgctggtcat cggagcagat tccaccaatt tggatccgga atctgttgat
     3121 gagatggagc ggcgcaagga gaaaatcatg ctgctgtctt tgcaacgtcg ccagcaacag
     3181 gaggaggcga aggcgcgcaa agagattgag gcttcccaga agcgagaaaa ggagcgcgaa
     3241 aaggaggagg aacgagcgcg gaaaaaggag gagcaaatgg cacggcgagc ggccattttg
     3301 gagcagcaca gactcaagaa agccattgaa gaggccgagc gagagggtaa aaccctggat
     3361 cggcccgatc tgcacgtgaa gctgcaatcg cattcatcca cctcaacgac cccacgactg
     3421 aggcagcagc gcaccacgcg tcccaggcct aagacaattc acgtggacga tgccagcgtg
     3481 gacatcagcg aggcttcaag catctctagt cggggcaaaa aaggctcaag ctcgaatcta
     3541 acctga
//
