LOCUS       sequence-92021-e       15261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ArchT-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15261)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4ArchT-EGFP
FEATURES             Location/Qualifiers
     source          1..15261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5454..5456,5457..6170)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5454..5456,5457..6167)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5496..5517)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5757..5776)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6104..6125
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7922..7933
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12933..12951)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12947..12994
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13017..13033)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13017..13033)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13070..13090)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13070..13088)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13109..13125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13109..13125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13122..13144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13133..13149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13157..13163,13164..13181,13182..13187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13202..13223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13340..13357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13511..14099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13591..13610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14270..15061,15062..15130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14893..14912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15131..15235)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggac cccatcgctc tgcaggcagg atacgacctg ctgggcgacg
     4741 gaaggccaga gaccctgtgg ctgggaatcg gaaccctgct gatgctgatc ggcaccttct
     4801 acttcatcgt gaagggctgg ggcgtgaccg acaaggaggc cagggagtac tacagcatca
     4861 caatcctggt gcccggcatc gccagcgccg cctacctgag catgttcttc ggcatcggcc
     4921 tgaccgaggt gaccgtggcc ggcgaggtgc tggacatcta ctacgccaga tacgccgact
     4981 ggctgttcac cacccctttg cttctgctcg acctggccct gctggctaag gtggacaggg
     5041 tgagcatcgg aaccctggtg ggagtggacg ccctgatgat cgtgaccggc ctgatcggcg
     5101 ccctgagcca caccccactg gctaggtaca gctggtggct gttcagcacc atctgcatga
     5161 tcgtggtgct gtacttcctg gctaccagcc tgagggctgc tgctaaggag aggggaccag
     5221 aggtggctag caccttcaac accctgaccg ccctggtgct ggtgctgtgg accgcctacc
     5281 ccatcctgtg gatcatcgga accgagggag ctggagtggt gggactggga atcgagaccc
     5341 tgctgttcat ggtgctggac gtgaccgcca aagtgggctt cggcttcatc ctgctgagga
     5401 gcagggccat cctgggcgac accgaggccc ccgagccccg accggtagta gcagtgagca
     5461 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5521 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5581 ccctgaagtt catttgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5641 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5701 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5761 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5821 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5881 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5941 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     6001 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6061 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6121 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa tgacccgggt
     6181 tataaagtgc tgggtcatct aagcttttca gtcgagaaaa aaacattaga tcagaagaac
     6241 aactggcaac acttctcaac ctgagactta cttcaagatg ctcgatcctg gagaggtcta
     6301 tgatgaccct attgacccaa tcgagttaga ggctgaaccc agaggaaccc ccattgtccc
     6361 caacatcttg aggaactctg actacaatct caactctcct ttgatagaag atcctgctag
     6421 actaatgtta gaatggttaa aaacagggaa tagaccttat cggatgactc taacagacaa
     6481 ttgctccagg tctttcagag ttttgaaaga ttatttcaag aaggtagatt tgggttctct
     6541 caaggtgggc ggaatggctg cacagtcaat gatttctctc tggttatatg gtgcccactc
     6601 tgaatccaac aggagccgga gatgtataac agacttggcc catttctatt ccaagtcgtc
     6661 ccccatagag aagctgttga atctcacgct aggaaataga gggctgagaa tccccccaga
     6721 gggagtgtta agttgccttg agagggttga ttatgataat gcatttggaa ggtatcttgc
     6781 caacacgtat tcctcttact tgttcttcca tgtaatcacc ttatacatga acgccctaga
     6841 ctgggatgaa gaaaagacca tcctagcatt atggaaagat ttaacctcag tggacatcgg
     6901 gaaggacttg gtaaagttca aagaccaaat atggggactg ctgatcgtga caaaggactt
     6961 tgtttactcc caaagttcca attgtctttt tgacagaaac tacacactta tgctaaaaga
     7021 tcttttcttg tctcgcttca actccttaat ggtcttgctc tctcccccag agccccgata
     7081 ctcagatgac ttgatatctc aactatgcca gctgtacatt gctggggatc aagtcttgtc
     7141 tatgtgtgga aactccggct atgaagtcat caaaatattg gagccatatg tcgtgaatag
     7201 tttagtccag agagcagaaa agtttaggcc tctcattcat tccttgggag actttcctgt
     7261 atttataaaa gacaaggtaa gtcaacttga agagacgttc ggtccctgtg caagaaggtt
     7321 ctttagggct ctggatcaat tcgacaacat acatgacttg gtttttgtgt ttggctgtta
     7381 caggcattgg gggcacccat atatagatta tcgaaagggt ctgtcaaaac tatatgatca
     7441 ggttcacctt aaaaaaatga tagataagtc ctaccaggag tgcttagcaa gcgacctagc
     7501 caggaggatc cttagatggg gttttgataa gtactccaag tggtatctgg attcaagatt
     7561 cctagcccga gaccacccct tgactcctta tatcaaaacc caaacatggc cacccaaaca
     7621 tattgtagac ttggtggggg atacatggca caagctcccg atcacgcaga tctttgagat
     7681 tcctgaatca atggatccgt cagaaatatt ggatgacaaa tcacattctt tcaccagaac
     7741 gagactagct tcttggctgt cagaaaaccg aggggggcct gttcctagcg aaaaagttat
     7801 tatcacggcc ctgtctaagc cgcctgtcaa tccccgagag tttctgaggt ctatagacct
     7861 cggaggattg ccagatgaag acttgataat tggcctcaag ccaaaggaac gggaattgaa
     7921 gattgaaggt cgattctttg ctctaatgtc atggaatcta agattgtatt ttgtcatcac
     7981 tgaaaaactc ttggccaact acatcttgcc actttttgac gcgctgacta tgacagacaa
     8041 cctgaacaag gtgtttaaaa agctgatcga cagggtcacc gggcaagggc ttttggacta
     8101 ttcaagggtc acatatgcat ttcacctgga ctatgaaaag tggaacaacc atcaaagatt
     8161 agagtcaaca gaggatgtat tttctgtcct agatcaagtg tttggattga agagagtgtt
     8221 ttctagaaca cacgagtttt ttcaaaaggc ctggatctat tattcagaca gatcagacct
     8281 catcgggtta cgggaggatc aaatatactg cttagatgcg tccaacggcc caacctgttg
     8341 gaatggccag gatggcgggc tagaaggctt acggcagaag ggctggagtc tagtcagctt
     8401 attgatgata gatagagaat ctcaaatcag gaacacaaga accaaaatac tagctcaagg
     8461 agacaaccag gttttatgtc cgacatacat gttgtcgcca gggctatctc aagaggggct
     8521 cctctatgaa ttggagagaa tatcaaggaa tgcactttcg atatacagag ccgtcgagga
     8581 aggggcatct aagctagggc tgatcatcaa gaaagaagag accatgtgta gttatgactt
     8641 cctcatctat ggaaaaaccc ctttgtttag aggtaacata ttggtgcctg agtccaaaag
     8701 atgggccaga gtctcttgcg tctctaatga ccaaatagtc aacctcgcca atataatgtc
     8761 gacagtgtcc accaatgcgc taacagtggc acaacactct caatctttga tcaaaccgat
     8821 gagggatttt ctgctcatgt cagtacaggc agtctttcac tacctgctat ttagcccaat
     8881 cttaaaggga agagtttaca agattctgag cgctgaaggg gagagctttc tcctagccat
     8941 gtcaaggata atctatctag atccttcttt gggagggata tctggaatgt ccctcggaag
     9001 attccatata cgacagttct cagaccctgt ctctgaaggg ttatccttct ggagagagat
     9061 ctggttaagc tcccaagagt cctggattca cgcgttgtgt caagaggctg gaaacccaga
     9121 tcttggagag agaacactcg agagcttcac tcgccttcta gaagatccga ccaccttaaa
     9181 tatcagagga ggggccagtc ctaccattct actcaaggat gcaatcagaa aggctttata
     9241 tgacgaggtg gacaaggtgg aaaattcaga gtttcgagag gcaatcctgt tgtccaagac
     9301 ccatagagat aattttatac tcttcttaat atctgttgag cctctgtttc ctcgatttct
     9361 cagtgagcta ttcagttcgt cttttttggg aatccccgag tcaatcattg gattgataca
     9421 aaactcccga acgataagaa ggcagtttag aaagagtctc tcaaaaactt tagaagaatc
     9481 cttctacaac tcagagatcc acgggattag tcggatgacc cagacacctc agagggttgg
     9541 gggggtgtgg ccttgctctt cagagagggc agatctactt agggagatct cttggggaag
     9601 aaaagtggta ggcacgacag ttcctcaccc ttctgagatg ttgggattac ttcccaagtc
     9661 ctctatttct tgcacttgtg gagcaacagg aggaggcaat cctagagttt ctgtatcagt
     9721 actcccgtcc tttgatcagt catttttttc acgaggcccc ctaaagggat acttgggctc
     9781 gtccacctct atgtcgaccc agctattcca tgcatgggaa aaagtcacta atgttcatgt
     9841 ggtgaagaga gctctatcgt taaaagaatc tataaactgg ttcattacta gagattccaa
     9901 cttggctcaa gctctaatta ggaacattat gtctctgaca ggccctgatt tccctctaga
     9961 ggaggcccct gtcttcaaaa ggacggggtc agccttgcat aggttcaagt ctgccagata
    10021 cagcgaagga gggtattctt ctgtctgccc gaacctcctc tctcatattt ctgttagtac
    10081 agacaccatg tctgatttga cccaagacgg gaagaactac gatttcatgt tccagccatt
    10141 gatgctttat gcacagacat ggacatcaga gctggtacag agagacacaa ggctaagaga
    10201 ctctacgttt cattggcacc tccgatgcaa caggtgtgtg agacccattg acgacgtgac
    10261 cctggagacc tctcagatct tcgagtttcc ggatgtgtcg aaaagaatat ccagaatggt
    10321 ttctggggct gtgcctcact tccagaggct tcccgatatc cgtctgagac caggagattt
    10381 tgaatctcta agcggtagag aaaagtctca ccatatcgga tcagctcagg ggctcttata
    10441 ctcaatctta gtggcaattc acgactcagg atacaatgat ggaaccatct tccctgtcaa
    10501 catatacggc aaggtttccc ctagagacta tttgagaggg ctcgcaaggg gagtattgat
    10561 aggatcctcg atttgcttct tgacaagaat gacaaatatc aatattaata gacctcttga
    10621 attggtctca ggggtaatct catatattct cctgaggcta gataaccatc cctccttgta
    10681 cataatgctc agagaaccgt ctcttagagg agagatattt tctatccctc agaaaatccc
    10741 cgccgcttat ccaaccacta tgaaagaagg caacagatca atcttgtgtt atctccaaca
    10801 tgtgctacgc tatgagcgag agataatcac ggcgtctcca gagaatgact ggctatggat
    10861 cttttcagac tttagaagtg ccaaaatgac gtacctatcc ctcattactt accagtctca
    10921 tcttctactc cagagggttg agagaaacct atctaagagt atgagagata acctgcgaca
    10981 attgagttct ttgatgaggc aggtgctggg cgggcacgga gaagatacct tagagtcaga
    11041 cgacaacatt caacgactgc taaaagactc tttacgaagg acaagatggg tggatcaaga
    11101 ggtgcgccat gcagctagaa ccatgactgg agattacagc cccaacaaga aggtgtcccg
    11161 taaggtagga tgttcagaat gggtctgctc tgctcaacag gttgcagtct ctacctcagc
    11221 aaacccggcc cctgtctcgg agcttgacat aagggccctc tctaagaggt tccagaaccc
    11281 tttgatctcg ggcttgagag tggttcagtg ggcaaccggt gctcattata agcttaagcc
    11341 tattctagat gatctcaatg ttttcccatc tctctgcctt gtagttgggg acgggtcagg
    11401 ggggatatca agggcagtcc tcaacatgtt tccagatgcc aagcttgtgt tcaacagtct
    11461 tttagaggtg aatgacctga tggcttccgg aacacatcca ctgcctcctt cagcaatcat
    11521 gaggggagga aatgatatcg tctccagagt gatagatctt gactcaatct gggaaaaacc
    11581 gtccgacttg agaaacttgg caacctggaa atacttccag tcagtccaaa agcaggtcaa
    11641 catgtcctat gacctcatta tttgcgatgc agaagttact gacattgcat ctatcaaccg
    11701 gatcaccctg ttaatgtccg attttgcatt gtctatagat ggaccactct atttggtctt
    11761 caaaacttat gggactatgc tagtaaatcc aaactacaag gctattcaac acctgtcaag
    11821 agcgttcccc tcggtcacag ggtttatcac ccaagtaact tcgtcttttt catctgagct
    11881 ctacctccga ttctccaaac gagggaagtt tttcagagat gctgagtact tgacctcttc
    11941 cacccttcga gaaatgagcc ttgtgttatt caattgtagc agccccaaga gtgagatgca
    12001 gagagctcgt tccttgaact atcaggatct tgtgagagga tttcctgaag aaatcatatc
    12061 aaatccttac aatgagatga tcataactct gattgacagt gatgtagaat cttttctagt
    12121 ccacaagatg gttgatgatc ttgagttaca gaggggaact ctgtctaaag tggctatcat
    12181 tatagccatc atgatagttt tctccaacag agtcttcaac gtttccaaac ccctaactga
    12241 cccctcgttc tatccaccgt ctgatcccaa aatcctgagg cacttcaaca tatgttgcag
    12301 tactatgatg tatctatcta ctgctttagg tgacgtccct agcttcgcaa gacttcacga
    12361 cctgtataac agacctataa cttattactt cagaaagcaa gtcattcgag ggaacgttta
    12421 tctatcttgg agttggtcca acgacacctc agtgttcaaa agggtagcct gtaattctag
    12481 cctgagtctg tcatctcact ggatcaggtt gatttacaag atagtgaaga ctaccagact
    12541 cgttggcagc atcaaggatc tatccagaga agtggaaaga caccttcata ggtacaacag
    12601 gtggatcacc ctagaggata tcagatctag atcatcccta ctagactaca gttgcctgtg
    12661 aaccggatac tcctggaagc ctgcccatgc taagactctt gtgtgatgta tcttgaaaaa
    12721 aacaagatcc taaatctgaa cctttggttg tttgattgtt tttctcattt ttgttgttta
    12781 tttgttaagc gtgggtcggc atggcatctc cacctcctcg cggtccgacc tgggcatccg
    12841 aaggaggacg cacgtccact cggatggcta agggaggggc ggggatccgg ctgctaacaa
    12901 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
    12961 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc
    13021 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
    13081 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    13141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    13201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    13261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    13321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    13501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    13561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    13621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    13681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    13741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    13801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    13861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    13921 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    13981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    14041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    14101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    14161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    14221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    14281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    14341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    14401 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    14461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    14521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    14581 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    14641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    14701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    14761 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    14821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    14881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    14941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    15001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    15061 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    15121 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    15181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    15241 ttccccgaaa agtgccacct g
//
