LOCUS       sequence-92022-e       15252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ArchT-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15252)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4ArchT-mCherry
FEATURES             Location/Qualifiers
     source          1..15252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5454..6161
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5596..5614)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5864..5883
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7913..7924
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12924..12942)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12938..12985
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13008..13024)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13008..13024)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13061..13081)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13061..13079)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13100..13116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13100..13116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13113..13135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13124..13140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13148..13154,13155..13172,13173..13178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13193..13214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13331..13348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13502..14090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13582..13601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14261..15052,15053..15121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14884..14903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15122..15226)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggac cccatcgctc tgcaggcagg atacgacctg ctgggcgacg
     4741 gaaggccaga gaccctgtgg ctgggaatcg gaaccctgct gatgctgatc ggcaccttct
     4801 acttcatcgt gaagggctgg ggcgtgaccg acaaggaggc cagggagtac tacagcatca
     4861 caatcctggt gcccggcatc gccagcgccg cctacctgag catgttcttc ggcatcggcc
     4921 tgaccgaggt gaccgtggcc ggcgaggtgc tggacatcta ctacgccaga tacgccgact
     4981 ggctgttcac cacccctttg cttctgctcg acctggccct gctggctaag gtggacaggg
     5041 tgagcatcgg aaccctggtg ggagtggacg ccctgatgat cgtgaccggc ctgatcggcg
     5101 ccctgagcca caccccactg gctaggtaca gctggtggct gttcagcacc atctgcatga
     5161 tcgtggtgct gtacttcctg gctaccagcc tgagggctgc tgctaaggag aggggaccag
     5221 aggtggctag caccttcaac accctgaccg ccctggtgct ggtgctgtgg accgcctacc
     5281 ccatcctgtg gatcatcgga accgagggag ctggagtggt gggactggga atcgagaccc
     5341 tgctgttcat ggtgctggac gtgaccgcca aagtgggctt cggcttcatc ctgctgagga
     5401 gcagggccat cctgggcgac accgaggccc ccgagccccg accggtagta gcagtgagca
     5461 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5521 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5581 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5641 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5701 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5761 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     5821 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     5881 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     5941 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6001 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6061 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6121 gccgccactc taccggcggc atggacgagc tgtacaagta atgacccggg ttataaagtg
     6181 ctgggtcatc taagcttttc agtcgagaaa aaaacattag atcagaagaa caactggcaa
     6241 cacttctcaa cctgagactt acttcaagat gctcgatcct ggagaggtct atgatgaccc
     6301 tattgaccca atcgagttag aggctgaacc cagaggaacc cccattgtcc ccaacatctt
     6361 gaggaactct gactacaatc tcaactctcc tttgatagaa gatcctgcta gactaatgtt
     6421 agaatggtta aaaacaggga atagacctta tcggatgact ctaacagaca attgctccag
     6481 gtctttcaga gttttgaaag attatttcaa gaaggtagat ttgggttctc tcaaggtggg
     6541 cggaatggct gcacagtcaa tgatttctct ctggttatat ggtgcccact ctgaatccaa
     6601 caggagccgg agatgtataa cagacttggc ccatttctat tccaagtcgt cccccataga
     6661 gaagctgttg aatctcacgc taggaaatag agggctgaga atccccccag agggagtgtt
     6721 aagttgcctt gagagggttg attatgataa tgcatttgga aggtatcttg ccaacacgta
     6781 ttcctcttac ttgttcttcc atgtaatcac cttatacatg aacgccctag actgggatga
     6841 agaaaagacc atcctagcat tatggaaaga tttaacctca gtggacatcg ggaaggactt
     6901 ggtaaagttc aaagaccaaa tatggggact gctgatcgtg acaaaggact ttgtttactc
     6961 ccaaagttcc aattgtcttt ttgacagaaa ctacacactt atgctaaaag atcttttctt
     7021 gtctcgcttc aactccttaa tggtcttgct ctctccccca gagccccgat actcagatga
     7081 cttgatatct caactatgcc agctgtacat tgctggggat caagtcttgt ctatgtgtgg
     7141 aaactccggc tatgaagtca tcaaaatatt ggagccatat gtcgtgaata gtttagtcca
     7201 gagagcagaa aagtttaggc ctctcattca ttccttggga gactttcctg tatttataaa
     7261 agacaaggta agtcaacttg aagagacgtt cggtccctgt gcaagaaggt tctttagggc
     7321 tctggatcaa ttcgacaaca tacatgactt ggtttttgtg tttggctgtt acaggcattg
     7381 ggggcaccca tatatagatt atcgaaaggg tctgtcaaaa ctatatgatc aggttcacct
     7441 taaaaaaatg atagataagt cctaccagga gtgcttagca agcgacctag ccaggaggat
     7501 ccttagatgg ggttttgata agtactccaa gtggtatctg gattcaagat tcctagcccg
     7561 agaccacccc ttgactcctt atatcaaaac ccaaacatgg ccacccaaac atattgtaga
     7621 cttggtgggg gatacatggc acaagctccc gatcacgcag atctttgaga ttcctgaatc
     7681 aatggatccg tcagaaatat tggatgacaa atcacattct ttcaccagaa cgagactagc
     7741 ttcttggctg tcagaaaacc gaggggggcc tgttcctagc gaaaaagtta ttatcacggc
     7801 cctgtctaag ccgcctgtca atccccgaga gtttctgagg tctatagacc tcggaggatt
     7861 gccagatgaa gacttgataa ttggcctcaa gccaaaggaa cgggaattga agattgaagg
     7921 tcgattcttt gctctaatgt catggaatct aagattgtat tttgtcatca ctgaaaaact
     7981 cttggccaac tacatcttgc cactttttga cgcgctgact atgacagaca acctgaacaa
     8041 ggtgtttaaa aagctgatcg acagggtcac cgggcaaggg cttttggact attcaagggt
     8101 cacatatgca tttcacctgg actatgaaaa gtggaacaac catcaaagat tagagtcaac
     8161 agaggatgta ttttctgtcc tagatcaagt gtttggattg aagagagtgt tttctagaac
     8221 acacgagttt tttcaaaagg cctggatcta ttattcagac agatcagacc tcatcgggtt
     8281 acgggaggat caaatatact gcttagatgc gtccaacggc ccaacctgtt ggaatggcca
     8341 ggatggcggg ctagaaggct tacggcagaa gggctggagt ctagtcagct tattgatgat
     8401 agatagagaa tctcaaatca ggaacacaag aaccaaaata ctagctcaag gagacaacca
     8461 ggttttatgt ccgacataca tgttgtcgcc agggctatct caagaggggc tcctctatga
     8521 attggagaga atatcaagga atgcactttc gatatacaga gccgtcgagg aaggggcatc
     8581 taagctaggg ctgatcatca agaaagaaga gaccatgtgt agttatgact tcctcatcta
     8641 tggaaaaacc cctttgttta gaggtaacat attggtgcct gagtccaaaa gatgggccag
     8701 agtctcttgc gtctctaatg accaaatagt caacctcgcc aatataatgt cgacagtgtc
     8761 caccaatgcg ctaacagtgg cacaacactc tcaatctttg atcaaaccga tgagggattt
     8821 tctgctcatg tcagtacagg cagtctttca ctacctgcta tttagcccaa tcttaaaggg
     8881 aagagtttac aagattctga gcgctgaagg ggagagcttt ctcctagcca tgtcaaggat
     8941 aatctatcta gatccttctt tgggagggat atctggaatg tccctcggaa gattccatat
     9001 acgacagttc tcagaccctg tctctgaagg gttatccttc tggagagaga tctggttaag
     9061 ctcccaagag tcctggattc acgcgttgtg tcaagaggct ggaaacccag atcttggaga
     9121 gagaacactc gagagcttca ctcgccttct agaagatccg accaccttaa atatcagagg
     9181 aggggccagt cctaccattc tactcaagga tgcaatcaga aaggctttat atgacgaggt
     9241 ggacaaggtg gaaaattcag agtttcgaga ggcaatcctg ttgtccaaga cccatagaga
     9301 taattttata ctcttcttaa tatctgttga gcctctgttt cctcgatttc tcagtgagct
     9361 attcagttcg tcttttttgg gaatccccga gtcaatcatt ggattgatac aaaactcccg
     9421 aacgataaga aggcagttta gaaagagtct ctcaaaaact ttagaagaat ccttctacaa
     9481 ctcagagatc cacgggatta gtcggatgac ccagacacct cagagggttg ggggggtgtg
     9541 gccttgctct tcagagaggg cagatctact tagggagatc tcttggggaa gaaaagtggt
     9601 aggcacgaca gttcctcacc cttctgagat gttgggatta cttcccaagt cctctatttc
     9661 ttgcacttgt ggagcaacag gaggaggcaa tcctagagtt tctgtatcag tactcccgtc
     9721 ctttgatcag tcattttttt cacgaggccc cctaaaggga tacttgggct cgtccacctc
     9781 tatgtcgacc cagctattcc atgcatggga aaaagtcact aatgttcatg tggtgaagag
     9841 agctctatcg ttaaaagaat ctataaactg gttcattact agagattcca acttggctca
     9901 agctctaatt aggaacatta tgtctctgac aggccctgat ttccctctag aggaggcccc
     9961 tgtcttcaaa aggacggggt cagccttgca taggttcaag tctgccagat acagcgaagg
    10021 agggtattct tctgtctgcc cgaacctcct ctctcatatt tctgttagta cagacaccat
    10081 gtctgatttg acccaagacg ggaagaacta cgatttcatg ttccagccat tgatgcttta
    10141 tgcacagaca tggacatcag agctggtaca gagagacaca aggctaagag actctacgtt
    10201 tcattggcac ctccgatgca acaggtgtgt gagacccatt gacgacgtga ccctggagac
    10261 ctctcagatc ttcgagtttc cggatgtgtc gaaaagaata tccagaatgg tttctggggc
    10321 tgtgcctcac ttccagaggc ttcccgatat ccgtctgaga ccaggagatt ttgaatctct
    10381 aagcggtaga gaaaagtctc accatatcgg atcagctcag gggctcttat actcaatctt
    10441 agtggcaatt cacgactcag gatacaatga tggaaccatc ttccctgtca acatatacgg
    10501 caaggtttcc cctagagact atttgagagg gctcgcaagg ggagtattga taggatcctc
    10561 gatttgcttc ttgacaagaa tgacaaatat caatattaat agacctcttg aattggtctc
    10621 aggggtaatc tcatatattc tcctgaggct agataaccat ccctccttgt acataatgct
    10681 cagagaaccg tctcttagag gagagatatt ttctatccct cagaaaatcc ccgccgctta
    10741 tccaaccact atgaaagaag gcaacagatc aatcttgtgt tatctccaac atgtgctacg
    10801 ctatgagcga gagataatca cggcgtctcc agagaatgac tggctatgga tcttttcaga
    10861 ctttagaagt gccaaaatga cgtacctatc cctcattact taccagtctc atcttctact
    10921 ccagagggtt gagagaaacc tatctaagag tatgagagat aacctgcgac aattgagttc
    10981 tttgatgagg caggtgctgg gcgggcacgg agaagatacc ttagagtcag acgacaacat
    11041 tcaacgactg ctaaaagact ctttacgaag gacaagatgg gtggatcaag aggtgcgcca
    11101 tgcagctaga accatgactg gagattacag ccccaacaag aaggtgtccc gtaaggtagg
    11161 atgttcagaa tgggtctgct ctgctcaaca ggttgcagtc tctacctcag caaacccggc
    11221 ccctgtctcg gagcttgaca taagggccct ctctaagagg ttccagaacc ctttgatctc
    11281 gggcttgaga gtggttcagt gggcaaccgg tgctcattat aagcttaagc ctattctaga
    11341 tgatctcaat gttttcccat ctctctgcct tgtagttggg gacgggtcag gggggatatc
    11401 aagggcagtc ctcaacatgt ttccagatgc caagcttgtg ttcaacagtc ttttagaggt
    11461 gaatgacctg atggcttccg gaacacatcc actgcctcct tcagcaatca tgaggggagg
    11521 aaatgatatc gtctccagag tgatagatct tgactcaatc tgggaaaaac cgtccgactt
    11581 gagaaacttg gcaacctgga aatacttcca gtcagtccaa aagcaggtca acatgtccta
    11641 tgacctcatt atttgcgatg cagaagttac tgacattgca tctatcaacc ggatcaccct
    11701 gttaatgtcc gattttgcat tgtctataga tggaccactc tatttggtct tcaaaactta
    11761 tgggactatg ctagtaaatc caaactacaa ggctattcaa cacctgtcaa gagcgttccc
    11821 ctcggtcaca gggtttatca cccaagtaac ttcgtctttt tcatctgagc tctacctccg
    11881 attctccaaa cgagggaagt ttttcagaga tgctgagtac ttgacctctt ccacccttcg
    11941 agaaatgagc cttgtgttat tcaattgtag cagccccaag agtgagatgc agagagctcg
    12001 ttccttgaac tatcaggatc ttgtgagagg atttcctgaa gaaatcatat caaatcctta
    12061 caatgagatg atcataactc tgattgacag tgatgtagaa tcttttctag tccacaagat
    12121 ggttgatgat cttgagttac agaggggaac tctgtctaaa gtggctatca ttatagccat
    12181 catgatagtt ttctccaaca gagtcttcaa cgtttccaaa cccctaactg acccctcgtt
    12241 ctatccaccg tctgatccca aaatcctgag gcacttcaac atatgttgca gtactatgat
    12301 gtatctatct actgctttag gtgacgtccc tagcttcgca agacttcacg acctgtataa
    12361 cagacctata acttattact tcagaaagca agtcattcga gggaacgttt atctatcttg
    12421 gagttggtcc aacgacacct cagtgttcaa aagggtagcc tgtaattcta gcctgagtct
    12481 gtcatctcac tggatcaggt tgatttacaa gatagtgaag actaccagac tcgttggcag
    12541 catcaaggat ctatccagag aagtggaaag acaccttcat aggtacaaca ggtggatcac
    12601 cctagaggat atcagatcta gatcatccct actagactac agttgcctgt gaaccggata
    12661 ctcctggaag cctgcccatg ctaagactct tgtgtgatgt atcttgaaaa aaacaagatc
    12721 ctaaatctga acctttggtt gtttgattgt ttttctcatt tttgttgttt atttgttaag
    12781 cgtgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
    12841 gcacgtccac tcggatggct aagggagggg cggggatccg gctgctaaca aagcccgaaa
    12901 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
    12961 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat ccactagttc
    13021 tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
    13081 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    13141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    13201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    13261 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    13321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    13381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    13441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    13501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    13561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    13621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    13681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    13741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    13801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    13861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    13921 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    13981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    14041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    14101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    14161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    14221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    14281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    14341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    14401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    14461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    14521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    14581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    14641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    14701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    14761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    14821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    14881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    14941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    15001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    15061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    15121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    15181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    15241 aagtgccacc tg
//
